Microbeads on microposts: An inverted architecture for bead microarrays
| Autor: | Prashant D. Sawant, Dan V. Nicolau, Luisa Filipponi, Florin Fulga |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Silicon microstructures
Fabrication Materials science Silicon Protein Array Analysis Biomedical Engineering Biophysics chemistry.chemical_element Nanotechnology Biosensing Techniques Bead Sensitivity and Specificity Multiplexing Electrochemistry Dimethylpolysiloxanes technology industry and agriculture Reproducibility of Results Equipment Design General Medicine Microspheres Equipment Failure Analysis Replica molding chemistry visual_art visual_art.visual_art_medium DNA microarray Biotechnology Microfabrication |
| Zdroj: | Biosensors and Bioelectronics. 24:1850-1857 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2008.09.015 |
| Popis: | The rapid development of genomics and proteomics requires accelerated improvement of the microarrays density, multiplexing, readout capabilities and cost-effectiveness. The bead arrays are increasingly attractive because of their self-assembly-based fabrication, which alleviates many problems of top-down microfabrication. Here we present a simple, reliable, robust and modular technique for the fabrication of bead microarrays, which combines the directed assembling of beads in microstructures and PDMS-based replica molding. The beads are first self-assembled in pyramidal microwells fabricated by anisotropic etching of silicon substrates, then transferred on the apex of PDMS pyramids that replicate the silicon microstructures. The arrays are chemically and biochemically robust; they are spatially addressable and have the potential for being informationally addressable; and they appear to offer better readout capabilities than the classical microarrays. |
| Databáze: | OpenAIRE |
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