bta-miR-23a Regulates the Myogenic Differentiation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting MDFIC Gene

Autor: Lingyang Xu, Lupei Zhang, Qian Li, Xin Hu, Qiyuan Yang, Yishen Xing, Min Du, Ling Ren, Luc Willems, Junya Li, Yahui Wang
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Genes, Vol 11, Iss 1232, p 1232 (2020)
Genes
Volume 11
Issue 10
ISSN: 2073-4425
Popis: miR-23a, a member of the miR-23a/24-2/27a cluster, has been demonstrated to play pivotal roles in many cellular activities. However, the mechanisms of how bta-miR-23a controls the myogenic differentiation (MD) of PDGFR&alpha
&minus
bovine progenitor cells (bPCs) remain poorly understood. In the present work, bta-miR-23a expression was increased during the MD of PDGFR&alpha
bPCs. Moreover, bta-miR-23a overexpression significantly promoted the MD of PDGFR&alpha
bPCs. Luciferase reporter assays showed that the 3&rsquo
UTR region of MDFIC (MyoD family inhibitor domain containing) could be a promising target of bta-miR-23a, which resulted in its post-transcriptional down-regulation. Additionally, the knockdown of MDFIC by siRNA facilitated the MD of PDGFR&alpha
bPCs, while the overexpression of MDFIC inhibited the activating effect of bta-miR-23a during MD. Of note, MDFIC might function through the interaction between MyoG transcription factor and MEF2C promoter. This study reveals that bta-miR-23a can promote the MD of PDGFR&alpha
bPCs through post-transcriptional downregulation of MDFIC.
Databáze: OpenAIRE