Prion Protein Polymorphisms Affect Chronic Wasting Disease Progression
| Autor: | Debbie McKenzie, Camilo Duque-Velasquez, Joshua P. Vanderloo, Chad J. Johnson, Allen Herbst, Rick Chappell, Phil Bochsler |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Prions
animal diseases Animal Types Blotting Western lcsh:Medicine Biology Large Animals Microbiology Incubation period PRNP Prion Diseases Infectious Diseases of the Nervous System Neurobiology of Disease and Regeneration medicine Genetics Animals Genetic Predisposition to Disease Allele lcsh:Science Incubation Veterinary Prion Diseases Multidisciplinary Polymorphism Genetic Inoculation Deer lcsh:R Neurodegenerative Diseases Chronic wasting disease medicine.disease Virology Immunohistochemistry Glutamine Emerging Infectious Diseases Infectious Diseases Neurology Veterinary Diseases Glycine Genetics of Disease Disease Progression Medicine Wasting Disease Chronic lcsh:Q Veterinary Science Animal Genetics Research Article Neuroscience |
| Zdroj: | PLoS ONE PLoS ONE, Vol 6, Iss 3, p e17450 (2011) |
| ISSN: | 1932-6203 |
| Popis: | Analysis of the PRNP gene in cervids naturally infected with chronic wasting disease (CWD) suggested that PRNP polymorphisms affect the susceptibility of deer to infection. To test this effect, we orally inoculated 12 white-tailed deer with CWD agent. Three different PRNP alleles, wild-type (wt; glutamine at amino acid 95 and glycine at 96), Q95H (glutamine to histidine at amino acid position 95) and G96S (glycine to serine at position 96) were represented in the study cohort with 5 wt/wt, 3 wt/G96S, and 1 each wt/Q95H and Q95H/G96S. Two animals were lost to follow-up due to intercurrent disease. The inoculum was prepared from Wisconsin hunter-harvested homozygous wt/wt animals. All infected deer presented with clinical signs of CWD; the orally infected wt/wt had an average survival period of 693 days post inoculation (dpi) and G96S/wt deer had an average survival period of 956 dpi. The Q95H/wt and Q95H/G96S deer succumbed to CWD at 1,508 and 1,596 dpi respectively. These data show that polymorphisms in the PRNP gene affect CWD incubation period. Deer heterozygous for the PRNP alleles had extended incubation periods with the Q95H allele having the greatest effect. |
| Databáze: | OpenAIRE |
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