In VivoRadioprotection by 5-Androstenediol: Stimulation of the Innate Immune System
| Autor: | Cynthia E. Inal, Thomas M. Seed, Mark H. Whitnall, Venita Miner, Vilmar Villa, William E. Jackson |
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| Rok vydání: | 2001 |
| Předmět: |
Androstenediol
medicine.medical_specialty Cellular immunity Neutrophils Biophysics Macrophage-1 Antigen Radiation-Protective Agents Natural killer cell Leukocyte Count Mice chemistry.chemical_compound In vivo Internal medicine medicine Animals Radiology Nuclear Medicine and imaging Radiation Innate immune system biology Monocyte Flow Cytometry Endocrinology medicine.anatomical_structure Peroxidases Integrin alpha M chemistry Immunology Erythrocyte Count biology.protein Female Myelopoiesis Whole-Body Irradiation |
| Zdroj: | Radiation Research. 156:283-293 |
| ISSN: | 1938-5404 0033-7587 |
| Popis: | We showed previously that 5-androstenediol stimulates myelopoiesis, increases the numbers of circulating neutrophils and platelets, and enhances resistance to infection in gamma-irradiated mice. We have extended those studies to include monocytes, natural killer (NK) cells, eosinophils and basophils, and we have measured the activation marker CD11b using flow cytometry. Androstenediol (160 mg/kg) was administered subcutaneously to female B6D2F1 mice 24 h before whole-body gamma irradiation. Androstenediol treatments increased the blood levels of neutrophils, monocytes and NK cells in unirradiated animals; decreased the numbers of circulating eosinophils; and ameliorated radiation-induced decreases in neutrophils, monocytes, NK cells, erythrocytes and platelets. The androstenediol treatments had no significant effect on the numbers of circulating B cells or T cells. CD11b labeling intensity on monocytes was decreased slightly after androstenediol treatment. In contrast, radiation or androstenediol alone caused increases in CD11b labeling intensity on NK cells. Androstenediol and radiation combined caused a marked increase in NK cell CD11b. The results indicate that androstenediol increases the numbers of the three major cell types of the innate immune system (neutrophils, monocytes and NK cells), that androstenediol-induced changes in blood elements in irradiated animals persist for at least several weeks, and that there is a significant positive interaction between radiation and administration of androstenediol in the activation of NK cells. |
| Databáze: | OpenAIRE |
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