Downregulation of mir-23b in plasma is associated with poor prognosis in patients with colorectal cancer
| Autor: | Chang‑Hua Kou, Xi‑Lin Han, Hui‑Jie Zhuang, Tian Zhou, Hai‑Xin Qian |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oncology Cancer Research medicine.medical_specialty Oncogene business.industry Colorectal cancer Cancer Articles medicine.disease medicine.disease_cause Molecular medicine 03 medical and health sciences 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis Internal medicine Gene expression microRNA Cancer research Medicine Cancer biomarkers business Carcinogenesis |
| Popis: | MicroRNAs (miRNAs) are short, non-coding RNA molecules that act as regulators of gene expression. Circulating blood miRNAs have potential as cancer biomarkers. The main objective of the present study was to assess the effect of miRNA-23b (miR-23b) expression in plasma on the diagnosis and prognosis of colorectal cancer (CRC). Reverse transcription-quantitative polymerase chain reaction (PCR) was used to measure miR-23b expression levels, and methylation-specific PCR was used to test the promoter methylation status. Subsequently, the expression level of miR-23b in plasma samples was compared between CRC patients and healthy control individuals. The miR-23b expression levels were significantly lower in CRC cells and primary CRC tissues than in nonmalignant colorectal tissues (P |
| Databáze: | OpenAIRE |
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