Ribosome-inactivating proteins and other lectins from Adenia (Passifloraceae)

Autor: Chiara Lubelli, Letizia Polito, Luigi Barbieri, Andrea Bolognesi, Emanuele Pelosi, Fiorenzo Stirpe
Přispěvatelé: PELOSI E., LUBELLI C., POLITO L., BARBIERI L., BOLOGNESI A., STIRPE F.
Jazyk: angličtina
Rok vydání: 2005
Předmět:
Popis: The caudices of ten Adenia species contain galactose-binding lectins that were purified by affinity chromatography. All lectins but three agglutinate human erythrocytes. Six lectins consist of two unequal chains, which can be separated by reduction, and inhibit protein synthesis both by a rabbit reticulocyte lysate and by HeLa and Raji cells. The lectins from A. goetzii, A. lanceolata and A. stenodactyla had the highest cytotoxicity, inhibiting cell protein synthesis with IC50’s (concentration inhibiting by 50%) below 0.1 ng/ml, and deadenylate DNA, thus being type 2 ribosome-inactivating proteins.
Databáze: OpenAIRE