Continuous Transforming Growth Factor β1Secretion by Cell-Mediated Gene Therapy Maintains Chondrocyte Redifferentiation
| Autor: | Kyoung Baek Choi, Dug Keun Lee, Jeannie Kim, Chae-Lyul Lim, Guang Fan Chi, Eun Byul Lee, Kwan Hee Lee, In Suk Oh, Sally Hwang, Youngsuk Yi, Sun U. Song, Moon Jong Noh, Vivian Yip, Hyeon-Youl Lee, Jong-Pil Hyun |
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| Rok vydání: | 2005 |
| Předmět: |
Cartilage
Articular Cell Transplantation Transgene Genetic enhancement Cellular differentiation Genetic Vectors Transplantation Heterologous Type II collagen Mice Nude Biology Chondrocyte Viral vector Transforming Growth Factor beta1 Mice Chondrocytes Transforming Growth Factor beta medicine Animals Humans Transgenes Collagen Type II Cells Cultured Cartilage General Engineering Cell Differentiation Genetic Therapy Molecular biology Retroviridae medicine.anatomical_structure Feasibility Studies Transforming growth factor |
| Zdroj: | Tissue Engineering. 11:310-318 |
| ISSN: | 1557-8690 1076-3279 |
| DOI: | 10.1089/ten.2005.11.310 |
| Popis: | One of the most important factors in the production of cartilage is transforming growth factor beta1 (TGF-beta1). To obtain sustained release of TGF-beta1, a cell-mediated gene therapy technique was introduced. We infected chondrocytes with a retroviral vector carrying the TGF-beta1 gene. The single clone derivative showed sustained TGF-beta1 secretion. It also showed constitutive type II collagen expression. Whereas the TGF-beta1 protein itself is unable to induce formation of cartilage in vivo, human chondrocytes engineered to express a retroviral vector encoding TGF-beta1 showed cartilage formation in vivo when cells were injected into nude mice intradermally. These data suggest that cell-mediated gene therapy using TGF-beta1 as a transgene would be a promising treatment for osteoarthritis. |
| Databáze: | OpenAIRE |
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