The tomato CAROTENOID CLEAVAGE DIOXYGENASE8 (SlCCD8) regulates rhizosphere signaling, plant architecture and affects reproductive development through strigolactone biosynthesis

Autor: Harro J. Bouwmeester, R.A. de Maagd, T. Pollina, P. Toth, Tatsiana Charnikhova, Wouter Kohlen, Carolien Ruyter-Spira, Juan Antonio López-Ráez, Michiel Lammers, Imran Haider, María J. Pozo
Jazyk: angličtina
Rok vydání: 2012
Předmět:
0106 biological sciences
phosphate deficiency
Physiology
Apical dominance
Plant Science
Genetically modified crops
01 natural sciences
Plant Roots
Mass Spectrometry
Lactones
Solanum lycopersicum
Gene Expression Regulation
Plant
orobanche spp
Laboratorium voor Plantenfysiologie
Cloning
Molecular
Plant Proteins
0303 health sciences
Rhizosphere
biology
Reproduction
Plant physiology
food and beverages
Gene Knockdown Techniques
Seeds
Bioscience
RNA Interference
Plant Shoots
Laboratory of Plant Physiology
Signal Transduction
Plant Exudates
Molecular Sequence Data
Strigolactone
arbuscular mycorrhizal fungi
Flowers
Models
Biological
03 medical and health sciences
Botany
Amino Acid Sequence
RNA
Messenger
BIOS Plant Development Systems
mutant
gene
030304 developmental biology
rice
fungi
Xylem
biology.organism_classification
arabidopsis
germination
Plant morphology
Fruit
tiller bud outgrowth
parasitic plants
Solanum
010606 plant biology & botany
Chromatography
Liquid
Zdroj: New Phytologist, 196(2), 535-547
New Phytologist 196 (2012) 2
ISSN: 0028-646X
DOI: 10.1111/j.1469-8137.2012.04265.x
Popis: •Strigolactones are plant hormones that regulate both above- and belowground plant architecture. Strigolactones were initially identified as rhizosphere signaling molecules. In the present work, the tomato (Solanum lycopersicum) CAROTENOID CLEAVAGE DIOXYGENASE 8 (SlCCD8) was cloned and its role in rhizosphere signaling and plant physiology assessed by generating knock-down lines. •Transgenic SlCCD8 plants were generated by RNAi-mediated silencing. Lines with different levels of strigolactone reduction – confirmed by UPLC-MS/MS – were selected and their phenotypes investigated. •Lines exhibiting reduced SlCCD8 levels displayed increased shoot branching, reduced plant height, increased number of nodes and excessive adventitious root development. In addition, these lines exhibited reproductive phenotypes such as smaller flowers, fruits, as well as fewer and smaller seeds per fruit. Furthermore, we show that strigolactone loading to the xylem sap is possibly restricted to orobanchol. •Infestation by Phelipanche ramosa was reduced by 90% in lines with a relatively mild reduction in strigolactone biosynthesis and secretion while arbuscular mycorrhizal symbiosis, apical dominance and fruit yield were only mildly affected. This demonstrates that reduction of strigolactone biosynthesis could be a suitable tool in parasitic weed management. Furthermore, our results suggest that strigolactones are involved in even more physiological processes than so far assumed.
Databáze: OpenAIRE
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