Use of forward genetic screens to identify genes required for RNA-directed DNA methylation in Arabidopsis thaliana
| Autor: | Marjori Matzke, Changho Eun, Taku Sasaki, Zdravko J. Lorković, A. J. M. Matzke, Ulf Naumann |
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| Rok vydání: | 2012 |
| Předmět: |
Genetics
Transposable element biology Arabidopsis DNA Methylation biology.organism_classification Genes Plant Biochemistry Epigenetics of physical exercise RNA Plant DNA methylation Arabidopsis thaliana Epigenetics Gene Silencing Genetic Testing Molecular Biology RNA-Directed DNA Methylation Gene Genetic screen |
| Zdroj: | Cold Spring Harbor symposia on quantitative biology. 77 |
| ISSN: | 1943-4456 |
| Popis: | RNA-directed DNA methylation is a small RNA-mediated epigenetic modification that contributes to transcriptional silencing of transposons and repetitive sequences in plants. We have conducted several forward genetic screens to identify factors required for RNA-directed DNA methylation and transcriptional gene silencing in Arabidopsis thaliana. Here, we review the findings from these screens and report on two new mutants, dms12 and dms13, that are defective in Pol V–specific subunits NRPE5 and NRPE9b. Cumulative results from genetic screens performed in our laboratory and those of other investigators have revealed that RNA-directed DNA methylation requires a complex transcriptional machinery comprising a number of plant-specific factors, many of which were functionally uncharacterized before being implicated in this pathway. Future challenges include unraveling the detailed mechanism and full range of functions of RNA-directed DNA methylation. |
| Databáze: | OpenAIRE |
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