In vivo Imaging of the Cerebral Endothelial Glycocalyx in Mice
| Autor: | Jin-Hui Yoon, Eek-Sung Lee, Yong Jeong |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Wheat Germ Agglutinins Physiology Glycocalyx 03 medical and health sciences 0302 clinical medicine Venules Animals chemistry.chemical_classification biology Brain Endothelial Cells Cerebral Arteries Endothelial glycocalyx Cerebral Veins Capillaries Cell biology Mice Inbred C57BL Microscopy Fluorescence Multiphoton 030104 developmental biology Xanthenes Proteoglycan chemistry biology.protein Cardiology and Cardiovascular Medicine Glycoprotein Fluorescein-5-isothiocyanate 030217 neurology & neurosurgery Preclinical imaging |
| Zdroj: | Journal of Vascular Research. 54:59-67 |
| ISSN: | 1423-0135 1018-1172 |
| DOI: | 10.1159/000457799 |
| Popis: | Background/Aims: Endothelial glycocalyx refers to the proteoglycan or glycoprotein layer of vessel walls and has critical physiological functions. Cerebral glycocalyx may have additional functions considering the blood-brain barrier and other features. However, the assessment of it has only been performed ex vivo, which includes processes presumably damaging the glycocalyx layer. Here we visualize and characterize the cerebral endothelial glycocalyx in vivo. Methods: We visualized and quantified the cerebral endothelial glycocalyx in vivo under a 2-photon microscope by tagging glycocalyx and vessel lumen with wheat germ agglutinin lectin and dextran, respectively. The radial intensity was analyzed to measure the thickness of the cerebral endothelial glycocalyx in each vessel type. Results: Cerebral arteries and capillaries have an intact endothelial glycocalyx, but veins and venules do not. The thickness of the glycocalyx layer in pial arteries, penetrating arteries, and capillaries was different; however, it was not correlated with the vessel diameter within each vessel type. Conclusion: We characterized the distribution of the cerebral endothelial glycocalyx in vivo. Compared to the results from ex vivo studies, the layer is thicker, indicating that the layer may be damaged in ex vivo systems. We also observed an inhomogeneous cerebral endothelial glycocalyx distribution that might reflect the functional heterogeneity of the vessel type. |
| Databáze: | OpenAIRE |
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