Large Differences in Urinary Benzene Metabolite S-Phenylmercapturic Acid Quantitation: A Comparison of Five LC-MS-MS Methods
| Autor: | Deepak Bhandari, Steven G. Carmella, Jean-François Bienvenu, Chloe Biren, Benjamin C. Blount, Kristina Bello, Peyton Jacob, Brett A. Bowman, Menglan Chen, Andrew Willmore, Brandon M. Kenwood, Stephen S. Hecht, Denise S. Tevis, Eric Gaudreau, Víctor R. De Jesús, Jia Liu |
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| Rok vydání: | 2020 |
| Předmět: |
Health
Toxicology and Mutagenesis Urinary system Metabolite Urine Toxicology Tandem mass spectrometry 01 natural sciences Article Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Tandem Mass Spectrometry Occupational Exposure Lc ms ms Environmental Chemistry Humans Sample preparation 030212 general & internal medicine Benzene Chemical Health and Safety Chromatography 010401 analytical chemistry 0104 chemical sciences Acetylcysteine chemistry S-phenylmercapturic acid Biomarkers Chromatography Liquid |
| Zdroj: | J Anal Toxicol |
| ISSN: | 1945-2403 |
| Popis: | Benzene is a known genotoxic carcinogen linked to many hematological abnormalities. S-phenylmercapturic acid (PHMA, N-acetyl-S-(phenyl)-L-cysteine, CAS# 4775-80-8) is a urinary metabolite of benzene and is used as a biomarker to assess benzene exposure. Pre-S-phenylmercapturic acid (pre-PHMA) is a PHMA precursor that dehydrates to PHMA at acidic pH. Published analytical methods that measure urinary PHMA adjust urine samples to a wide range of pH values using several types of acid, potentially leading to highly variable results depending on the concentration of pre-PHMA in a sample. Information is lacking on the variation in sample preparation among laboratories regularly measuring PHMA and the effect of those differences on PHMA quantitation in human urine samples. To investigate the differences in PHMA quantitation, we conducted an inter-laboratory comparison that included the analysis of 50 anonymous human urine samples (25 self-identified smokers and 25 self-identified non-smokers), quality control samples and commercially available reference samples in five laboratories using different analytical methods. Observed urinary PHMA concentrations were proportionally higher at lower pH, and results for anonymous urine samples varied widely among the methods. The method with the neutral preparation pH yielded results about 60% lower than the method using the most acidic conditions. Samples spiked with PHMA showed little variation, suggesting that the variability in results in human urine samples across methods is driven by the acid-mediated conversion of pre-PHMA to PHMA. |
| Databáze: | OpenAIRE |
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