Lung-specific direct in vivo gene transfer with recombinant plasmid DNA
| Autor: | B. Shepard, Min-Fu Tsan, Julie E. White |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Male Pulmonary and Respiratory Medicine Physiology Genetic enhancement Genetic Vectors DNA Recombinant Cytomegalovirus Simian virus 40 Biology Gene delivery Transfection law.invention Rats Sprague-Dawley Plasmid law Physiology (medical) Animals Cationic liposome Promoter Regions Genetic Enhancer Genes Immediate-Early Lung Drug Carriers Reporter gene Insufflation Cell Biology Molecular biology Rats Trachea Enhancer Elements Genetic Genes Bacterial Liposomes Recombinant DNA Plasmids |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 268:L1052-L1056 |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.1995.268.6.l1052 |
| Popis: | A number of gene delivery methods have been developed to facilitate gene transfer into mammalian somatic cells in vivo. In this study, we demonstrated that tracheal insufflation of two recombinant plasmids containing a bacterial gene, chloramphenicol acetyltransferase (CAT), driven by the cytomegalovirus (CMV) immediate early promoter/enhancer, either alone or complexed to cationic liposomes, resulted in efficient and selective transfection of the lungs in rats. When the simian virus 40 (SV40) promoter/enhancer was used, there was no detectable transfection. Insufflation of plasmid DNA was as efficient as plasmid-liposome complexes in transfecting the lungs. Expression of the CAT gene in the lungs was noted as early as 1 day after insufflation of plasmid DNA alone or plasmid-liposome complexes, and lasted for > 21 days. In contrast, intravenous injection of plasmid alone or plasmid-liposome complexes did not result in transfection of the lungs. Because of its simplicity, without the potential adverse effect of any gene delivery systems, intratracheal delivery of recombinant plasmid DNA may have potential implication for lung-specific gene therapy. |
| Databáze: | OpenAIRE |
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