Exosomes derived from LPS-stimulated human thymic mesenchymal stromal cells enhance inflammation via thrombospondin-1
| Autor: | Xin Peng, Pingping Liu, Yun Li, Lei Sun, Xiaoyan Xuan, Fei Zhao, Qianru Li, Ying Du, Jing Li, Chen Tan, Peng Wang, Yun Sun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Lipopolysaccharides
endocrine system Immunology & Inflammation THP-1 Cells Biophysics Inflammation Thymus Gland Exosomes Biochemistry Exosome Thrombospondin 1 Immune system immune system diseases medicine Gene silencing exosome Humans thrombospondin-1 Molecular Biology Research Articles Gene Expression & Regulation Chemistry Stem Cells Macrophages Mesenchymal stem cell Thymic mesenchymal stem cells virus diseases Cell Differentiation Mesenchymal Stem Cells Cell Biology Th1 Cells Microvesicles Cell biology Up-Regulation Phenotype Cytokines Th17 Cells Tumor necrosis factor alpha medicine.symptom Inflammation Mediators |
| Zdroj: | Bioscience Reports |
| ISSN: | 1573-4935 0144-8463 |
| Popis: | Inflammatory response mediated by immune cells is either directly or indirectly regulated by mesenchymal stromal cells (MSCs). Accumulating evidence suggests that thrombospondin-1 (TSP-1) is highly expressed in response to inflammation. In this work, we isolated and identified human thymic mesenchymal stromal cells (tMSCs) and detected the expression of TSP-1. We found that tMSCs expressed TSP-1 and Poly (I:C) or LPS treatment promoted the expression of TSP-1. Further, we isolated and identified exosomes originating from tMSCs (MEXs). Notably, exosomes derived from LPS-pretreated tMSCs (MEXsLPS) promoted the polarization of macrophages to M1-like phenotype and IL-6, TNF-α secretion as well as the pro-inflammatory differentiation of CD4+T cells into Th17 cells. Upon silencing the expression of TSP-1 in tMSCs, the pro-inflammatory effects of MEXsLPS were suppressed. Therefore, these findings uncovered TSP-1 as the principal factor in MEXsLPS pro-inflammatory regulation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|