Three-Dimensional Bioprinting Using a Coaxial Needle with Viscous Inks in Bone Tissue Engineering - An In vitro Study
| Autor: | Elin Pernevik, Sanna Sämfors, Göran Kjeller, George K.B. Sándor, Lars Rasmusson, Java Walladbegi, Christian Schaefer, Paul Gatenholm |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Scaffold
bioinks Autologous tissue Adipose stem cells CELLINK in vitro viability Bone tissue engineering 03 medical and health sciences 0302 clinical medicine Medicine In vitro study Viability assay 030223 otorhinolaryngology Fibula bone business.industry Kirurgi 030206 dentistry Original Article - In vitro Study Surgery Oral Surgery Stem cell Coaxial business Biomedical engineering |
| Zdroj: | Annals of Maxillofacial Surgery |
| ISSN: | 2249-3816 2231-0746 |
| Popis: | Introduction: Vascularized autologous tissue grafts are considered “gold standard” for the management of larger bony defects in the craniomaxillofacial area. This modality does however carry limitations, such as the absolute requirement for healthy donor tissues and recipient vessels. In addition, the significant morbidity of large bone graft is deterrent to fibula bone flap use. Therefore, less morbid strategies would be beneficial. The purpose of this study was to develop a printing method to manufacture scaffold structure with viable stem cells. Materials and Methods: In total, three different combinations of ground beta tri-calcium phosphate and CELLINK (bioinks) were printed with a nozzle to identify a suitable bioink for three-dimensional printing. Subsequently, a coaxial needle, with three different nozzle gauge combinations, was evaluated for printing of the bioinks. Scaffold structures (grids) were then printed alone and with additional adipose stem cells before being transferred into an active medium and incubated overnight. Following incubation, grid stability was evaluated by assessing the degree of maintained grid outline, and cell viability was determined using the live/dead cell assay. Results: Among the three evaluated combinations of bioinks, two resulted in good printability for bioprinting. Adequate printing was obtained with two out of the three nozzle gauge combinations tested. However, due to the smaller total opening, one combination revealed a better stability. Intact grids with maintained stability were obtained using Ink B23 and Ink B42, and approximately 80% of the printed stem cells were viable following 24 hours. Discussion: Using a coaxial needle enables printing of a stable scaffold with viable stem cells. Furthermore, cell viability is maintained after the bioprinting process. |
| Databáze: | OpenAIRE |
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