Effect of hypobaria and hyperoxia during sepsis on survival and energy metabolism
Autor: | Julie L. Proctor, Pratistha Tamrakar, Myeongjin Choi, Turhan Coksaygan, Alan S. Cross, Gary Fiskum, Ashley Moore, Patricia F Schuck, Katrina Asbury |
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Rok vydání: | 2018 |
Předmět: |
Male
medicine.medical_specialty Sepsis mortality Energy metabolism Hyperoxia Critical Care and Intensive Care Medicine Medical care Mitochondria Heart Rats Sprague-Dawley Sepsis 03 medical and health sciences Oxygen Consumption 0302 clinical medicine Animals Medicine Heart metabolism business.industry Brain 030208 emergency & critical care medicine medicine.disease Mitochondria Rats Sprague dawley Disease Models Animal Atmospheric Pressure Emergency medicine Cytokines Surgery medicine.symptom Energy Metabolism business 030217 neurology & neurosurgery |
Zdroj: | Journal of Trauma and Acute Care Surgery. 85:S68-S76 |
ISSN: | 2163-0763 2163-0755 |
Popis: | Injured warfighters air evacuated to tertiary medical care facilities are subjected to many stresses that may promote the development of sepsis. In this study, we tested the hypothesis that exposure to "in-flight" hypobaria and/or hyperoxia within 24 hours after onset of intra-abdominal infection in rats accelerates the development and/or severity of sepsis and neurologic injury in survivors.Sprague-Dawley rats underwent cecal ligation/puncture (CLP) or sham procedures. Twenty-four hours later, rats were then placed in hypobaric chambers for 6 hours and assigned to normobaric conditions and maintained at either 21% or 100% O2, or under hypobaric conditions (pressure equivalent to an altitude of 8,000 ft) but maintained under either 28% or 100% O2. Two days after CLP or sham, blood samples were obtained for cytokine levels, and mitochondria were isolated from the brain and heart of a subset of animals for analysis of mitochondrial oxygen consumption. Animals were also evaluated for neuromotor impairment before and 15 days postsurgery.Among the 70 rats studied, 16.7% of CLP but none of the sham-treated rats died. All of the CLP but none of the sham rats had evidence of peritonitis at 2 days. Twenty percent (6 of 30) CLP rats undergoing hypobaria versus 12.5% (3 of 24) of CLP rats exposed to normobaria died (p = 0.715) while 12% (3 of 25) of CLP rats exposed to hyperoxia versus 20.7% (6 of 29) of CLP rats exposed to normoxia died (p = 0.48). The ratio of mitochondrial ATP-generating O2 consumption to resting respiration was higher in the CLP plus hypobaria under 100% compared with shams. The only difference in H2O2 production was observed in mitochondria from CLP rats exposed to hyperoxia under normobaric conditions. Composite neurologic scores obtained 15 days postinjury were lower than those at baseline for shams.We conclude that neither "in-flight" hyperoxia nor hypobaria exacerbate sepsis or neurologic injury. |
Databáze: | OpenAIRE |
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