Peptide-Guided Assembly of Repeat Protein Fragments
| Autor: | Oliver Zerbe, Andreas Plückthun, Erich Michel |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
chemistry.chemical_classification
0301 basic medicine Models Molecular biology Chemistry Protein design Proteins Peptide binding Target peptide Peptide General Chemistry General Medicine 010402 general chemistry 01 natural sciences Catalysis 0104 chemical sciences 03 medical and health sciences 030104 developmental biology Biochemistry Armadillo repeats biology.animal Armadillo Protein Fragment Single amino acid Peptides |
| Zdroj: | Angewandte Chemie (International ed. in English). 57(17) |
| ISSN: | 1521-3773 |
| Popis: | Herein, we present the peptide-guided assembly of complementary fragments of designed armadillo repeat proteins (dArmRPs) to create proteins that bind peptides not only with high affinity but also with good selectivity. We recently demonstrated that complementary N- and C-terminal fragments of dArmRPs form high-affinity complexes that resemble the structure of the full-length protein, and that these complexes bind their target peptides. We now demonstrate that dArmRPs can be split such that the fragments assemble only in the presence of a templating peptide, and that fragment mixtures enrich the combination with the highest affinity for this peptide. The enriched fragment combination discriminates single amino acid variations in the target peptide with high specificity. Our results suggest novel opportunities for the generation of new peptide binders by selection from dArmRP fragment mixtures. |
| Databáze: | OpenAIRE |
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