A Phage Tubulin Assembles Dynamic Filaments by an Atypical Mechanism to Center Viral DNA within the Host Cell
| Autor: | James A. Kraemer, Marcella L. Erb, Elizabeth A. Montabana, Duy Stephen L. Pham, Joe Pogliano, Katrina Nguyen, Hannah Wang, David A. Agard, Elena A. Zehr, Christopher A. Waddling |
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| Rok vydání: | 2012 |
| Předmět: |
Models
Molecular 1.1 Normal biological development and functioning Molecular Sequence Data Sequence alignment macromolecular substances Medical and Health Sciences Guanosine Diphosphate General Biochemistry Genetics and Molecular Biology Bacterial cell structure Bacteriophage Viral Proteins 03 medical and health sciences chemistry.chemical_compound Models Underpinning research Tubulin Microtubule Pseudomonas 2.1 Biological and endogenous factors Bacteriophages Viral Amino Acid Sequence Aetiology Cytoskeleton 030304 developmental biology 0303 health sciences biology 030306 microbiology Biochemistry Genetics and Molecular Biology(all) C-terminus Molecular DNA Biological Sciences biology.organism_classification Cell biology chemistry DNA Viral biology.protein Sequence Alignment Developmental Biology |
| Zdroj: | Cell, vol 149, iss 7 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/j.cell.2012.04.034 |
| Popis: | Summary Tubulins are essential for the reproduction of many eukaryotic viruses, but historically, bacteriophage were assumed not to require a cytoskeleton. Here, we identify a tubulin-like protein, PhuZ, from bacteriophage 201φ2-1 and show that it forms filaments in vivo and in vitro. The PhuZ structure has a conserved tubulin fold, with an unusual, extended C terminus that we demonstrate to be critical for polymerization in vitro and in vivo. Longitudinal packing in the crystal lattice mimics packing observed by EM of in-vitro-formed filaments, indicating how interactions between the C terminus and the following monomer drive polymerization. PhuZ forms a filamentous array that is required for positioning phage DNA within the bacterial cell. Correct positioning to the cell center and optimal phage reproduction only occur when the PhuZ filament is dynamic. Thus, we show that PhuZ assembles a spindle-like array that functions analogously to the microtubule-based spindles of eukaryotes. |
| Databáze: | OpenAIRE |
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