Development of a gold immunochromatographic strip for the rapid detection of 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in catfish

Autor:	Shanshan Song, Liqiang Liu, Hua Kuang, Lu Qianqian, Wu Xiaoling
Rok vydání:	2020
Předmět:	lcsh:Immunologic diseases. Allergy medicine.drug_class Metabolite Immunology Monoclonal antibody 01 natural sciences Rapid detection chemistry.chemical_compound 0404 agricultural biotechnology Furaltadone medicine immunoassay lcsh:Agriculture (General) amoz Chromatography medicine.diagnostic_test 010401 analytical chemistry 04 agricultural and veterinary sciences lcsh:S1-972 040401 food science 0104 chemical sciences catfish samples chemistry monoclonal antibody Immunoassay 3-amino-5-morpholinomethyl-2-oxazolidinone lcsh:RC581-607 Agronomy and Crop Science furaltadone Food Science Catfish
Zdroj:	Food and Agricultural Immunology, Vol 31, Iss 1, Pp 751-763 (2020)
ISSN:	1465-3443 0954-0105
DOI:	10.1080/09540105.2020.1771289
Popis:	In this study, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a gold immunochromatographic assay were developed to detect the furaltadone metabolite, 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ). The detection of AMOZ was based on the AMOZ derivative 3-([2-nitrophenyl] methyleneamino)-5-morpholinomethyl-2-oxazolidinone (2-NPAMOZ). The monoclonal anti-2-NPAMOZ antibody 4G11, was prepared and exhibited a 50%-inhibitory concentration (IC50) of 0.049 ng/mL. Its cross-reactivity with structurally related analogues was less than 3%. This immunochromatographic assay was assembled for the rapid screening of AMOZ in catfish samples. The strip assay had a cut-off value of 0.1 μg/kg and a limit of detection (LOD) of 0.009 μg/kg. Analysis of AMOZ in catfish indicated that the results from the strip assay were highly comparable to results obtained using ic-ELISA and LC-MS/MS. Therefore, the colloidal gold (CG) immunoassay is a sensitive screening method for the detection of AMOZ residues in catfish samples.
Databáze:	OpenAIRE
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