5S rRNA modification in the hyperthermophilic archaea Sulfolobus solfataricus and Pyrodictium occultum
| Autor: | H Mizushima, Karl O. Stetter, James A. McCloskey, Y Kuchino, Pamela F. Crain, Eveline Bruenger, Jeffrey A. Kowalak |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Hot Temperature
RNase P ved/biology.organism_classification_rank.species Molecular Sequence Data Cytidine Nucleic Acid Denaturation Biochemistry Sulfolobus 5S ribosomal RNA Genetics Ribonuclease T1 Molecular Biology biology Molecular mass Base Sequence ved/biology Thermophile Sulfolobus solfataricus RNA Ribosomal 5S Nucleosides Ribosomal RNA biology.organism_classification Archaea Nucleic acid Nucleic Acid Conformation Biotechnology |
| Zdroj: | FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 7(1) |
| ISSN: | 0892-6638 |
| Popis: | The 5S rRNAs from Sulfolobus solfataricus and Pyrodictium occultum were digested to nucleosides and analyzed using directly-combined HPLC/mass spectrometry. P. occultum 5S rRNA contains two modified nucleoside species, N4-acetylcytidine (ac4C) and N4-acetyl-2'-O-methylcytidine (ac4Cm). Oligonucleotides were generated from P. occultum 5S rRNA by RNase T1 hydrolysis, and their molecular weights were determined using electrospray mass spectrometry and compared with those predicted from the P. occultum 5S RNA gene sequence. Deviation in mass between expected and observed molecular weights permitted ac4Cm to be located at position 35, in the nonanucleotide CAA-CACC[ac4Cm]G, and the ac4C in one or both of two (C,U)G trinucleotides. 2'-O-Methylcytidine is unambiguously characterized in S. solfataricus 5S rRNA, confirming earlier tentative assignments at the analogous sequence position (Stahl, D.A., Luehrsen, K.R., Woese, C.R., and Pace, N.R. (1981) Nucleic Acids Res., Vol. 9, pp. 6129-6137; Dams, E., Londei, P., Cammarano, P., Vandenberghe, A., and De Wachter, R. (1983) Nucleic Acids Res. Vol. 11, pp. 4667-4676). Potential effects of the presence of ac4C and ac4Cm on thermal stabilization of 5S rRNA in thermophiles are discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text