Antibiotics-induced intestinal dysbacteriosis caused behavioral alternations and neuronal activation in different brain regions in mice
| Autor: | Pan Wang, Hao Zhang, Tao Chen, Ke Tu, Peng Cao, Yue-Fan Yang, Xiao-Jun Wu, Xin-Tong Qiu, Hui Yang, Mingming Zhang |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Neurology medicine.drug_class Antibiotics Gut flora Neuroprotection lcsh:RC346-429 Open field Mice 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Nociception assay Medicine Intestinal dysbacteriosis Molecular Biology lcsh:Neurology. Diseases of the nervous system Behavior biology business.industry Research Probiotics biology.organism_classification 030104 developmental biology Immunology Anxiety Psychopharmacology medicine.symptom business 030217 neurology & neurosurgery |
| Zdroj: | Molecular Brain Molecular Brain, Vol 14, Iss 1, Pp 1-10 (2021) |
| ISSN: | 1756-6606 |
| DOI: | 10.1186/s13041-021-00759-w |
| Popis: | Antibiotics affect gut microbial composition, leading to Gut-Brain-Axis imbalance and neurobehavioral changes. However, the intestinal dysbacteriosis associated behavior changes are not consistently reported. It is not clear whether these changes are transient or permanent. The neuroprotective effect of probiotics against intestinal dysbacteriosis induced alternations needs to be determined either. In the present study, oral antibiotic mixture including Ampicillin, Streptomycin, and Clindamycin was utilized to induce intestinal dysbacteriosis in mice. Antibiotics application triggered mechanical allodynia in von frey test and spontaneous pain in open field test. It also resulted in increased anxiety and depressive-like behaviors and damaged spatial memory performance. After application of probiotics, the mechanical allodynia and spontaneous pain were alleviated significantly. The anxiety behaviors, depressive-like behaviors and recognitive performance were ameliorative as well. By using Fos protein as a marker, it is found that the sensory, emotion and memory related brain regions were activated in mice with intestinal dysbacteriosis. Our study is not only helpful for enriching our basic knowledge for understanding the changed pain responses and related brain disorders in antibiotics-induced dysbacteriosis mice, but also beneficial for providing a more comprehensive mechanistic explanation for the regulation of antibiotics and probiotics on gut microbiota and relevant alternations in animal neurological behaviors. |
| Databáze: | OpenAIRE |
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