A Cost Effective Easy Competitive Enzyme-Linked Immunosorbent Assay Suitable for Monitoring Protective Immunity against the Rabies Virus in the Serum of Humans and Dogs
| Autor: | Eun-Sil Park, Pornsawan Pongsopawijit, Yada Aronthippaitoon, Kriangkrai Thongkorn, Thitima Atuntee, Akihito Kawai, Akira Noguchi, Hathairat Thananchai, Satoshi Inoue, Waraporn Samer, Wilaiwan Petsophonsakul |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Serum medicine.drug_class Cost-Benefit Analysis 030106 microbiology Enzyme-Linked Immunosorbent Assay medicine.disease_cause Monoclonal antibody Antibodies Viral 03 medical and health sciences 0302 clinical medicine Dogs Seroepidemiologic Studies Medicine Seroprevalence Animals Humans 030212 general & internal medicine Neutralizing antibody Antigens Viral biology business.industry Rabies virus Antibodies Monoclonal General Medicine medicine.disease Thailand Virology Infectious Diseases Immunization biology.protein Rabies Antibody business Conformational epitope |
| Zdroj: | Japanese journal of infectious diseases. 72(2) |
| ISSN: | 1884-2836 |
| Popis: | The coverage of rabies vaccinations has been reported at 70-80% of dogs in annual reports. However, there are still outbreaks of rabies among humans and dogs in Thailand, thus indicating the necessity of ensuring seroprevalence in vaccinated dogs and efficacy of human immunization. A cost effective easy competitive enzyme-linked immunosorbent assay (CEE-cELISA) was developed here for monitoring protective immunity against the rabies virus in human and dog serum samples using monoclonal antibody clone 1-46-12, which recognizes a conformational epitope of the rabies G protein. The ELISA plate is coated with the whole viral antigen from a commercial vaccine. The serotiter measured by the CEE-cELISA and by the gold standard assay (rapid fluorescent focus inhibition test), detecting the neutralizing antibody, showed a strong correlation, with an R value of 0.958 and 0.931 in humans and dogs, respectively. These correlations were detected in the serum samples from humans and dogs at antibody concentrations up to 100 and 10 IU/ml, respectively. This CEE-cELISA could be an alternative assay for evaluating mass rabies vaccination rapidly at a low cost as well as for detecting antirabies antibodies in the serum of not only humans but also other animal species. |
| Databáze: | OpenAIRE |
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