Antibiotics susceptibility of quinolones against Salmonella spp. strains isolated and molecularly sequenced for gyrA gene
| Autor: | Guadalupe Patricia Macías-Farrera, Esvieta Tenorio-Borroto, Humberto Gustavo Monroy-Salazar, Roberto Montes de Oca Jiménez, Fabiola Rivera-Ramírez, Jorge Antonio Varela-Guerrero, Gilberto Yong-Angel |
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| Rok vydání: | 2018 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Salmonella Nalidixic acid Swine 030106 microbiology Drug resistance Quinolones Biology medicine.disease_cause Microbiology Nalidixic Acid 03 medical and health sciences Anti-Infective Agents Disk Diffusion Antimicrobial Tests Ampicillin Drug Resistance Bacterial medicine Animals Base Sequence Sulfamethoxazole Salmonella enterica Carbenicillin biology.organism_classification Trimethoprim Anti-Bacterial Agents 030104 developmental biology Infectious Diseases DNA Gyrase Genes Bacterial Mutation Salmonella Infections Fluoroquinolones medicine.drug |
| Zdroj: | Microbial Pathogenesis. 114:286-290 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2017.11.067 |
| Popis: | Drug-resistant Salmonella is frequently detected in most parts of the world, and its rate of resistance has increased significantly in recent years. However, this study aimed to evaluate the minimum inhibitory concentration (MIC, determined with the Kirby-Bauer method) of quinolones in 86 Salmonella spp. strains isolated from pigs. Both the inside and outside of the QRDR region of strains were sequenced. The DNA sequence of the QRDR region of Salmonella spp. revealed the mutations S83F, D87N and S83Y. The region outside the QRDR showed a mutation in L582G. Forty-five isolates of Salmonella ssp. were categorized as quinolone-resistant; out of these, 16 corresponded to Salmonella enterica and isolates showed intermediate resistance (6.25%) to nalidixic acid. Three isolats (18.6%) were resistant to ampicillin; two (12.5%) were resistant to carbenicillin. Moreover, three (18.7%) isolates were resistant to gentamicin, nitrofurantoin and pefloxacin, and 8 (50%) were resistant to trimethoprim/sulfamethoxazole. Six percent of Salmonella spp. strains showed less resistance to antimicrobial agents compared to S. Thyphimurium (18%). The resistance to individual quinolones varied by serotypes. For S. anatum and S. Reading, it was 12.25%, and for S. choreaeaesuis, S. typhimurium monofasica, 6.25%. In contrast, S. agona, S. bredeney and S. london were sensitive to these antibiotics. In conclusion, quinolones have become the drugs of choice for the treatment of severe Salmonella infections. The study of mutations outside the QRDR region opens up new insights about the resistance of Salmonella to fluoroquinolones. |
| Databáze: | OpenAIRE |
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