Architecture of implanted bone matrix gelatin influences heterotopic calcification and new bone formation
| Autor: | Yoshio Yoshimura, Yasuyuk Horisaka, Kikuji Yamashita, Jun Kawada, Yasuo Okamoto, Naoyuki Matsumoto, Tomomichi Takagi |
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| Rok vydání: | 1991 |
| Předmět: |
Male
food.ingredient Transplantation Heterotopic chemistry.chemical_element Bone Matrix Calcium Gelatin General Biochemistry Genetics and Molecular Biology Bone and Bones food Calcification Physiologic medicine Animals Centrifugation Bone Development Demineralized bone matrix Skeletal muscle Osteoblast Rats Inbred Strains Anatomy medicine.disease Alkaline Phosphatase Rats medicine.anatomical_structure chemistry Alkaline phosphatase Biomedical engineering Calcification |
| Zdroj: | Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.). 197(3) |
| ISSN: | 0037-9727 |
| Popis: | Heterotopic bone formation in skeletal muscle induced by compacted demineralized bone matrix gelatin (BMG) was studied histologically and biochemically. BMG was obtained by dehydrating diaphyseal shafts of femora and tibiae of 4-week-old male Sprague-Dawley rats, cutting the bone into chips, and demineralizing and extracting the chips with various solutions. The BMG was treated with 4 M guanidine-HCl, and compacted BMG was prepared by centrifugation. The compacted BMG was implanted into the rectus abdominis muscle of 5-week-old male Sprague-Dawley rats. The resulting specimens were examined histologically, and their alkaline phosphatase activity and the calcium content of the tissues were measured 3, 5, 7, 10, and 15 days after implantation. The BMG (separated BMG) with 75- to 500-microns particle sizes were implanted into control rats. The results showed that calcification, alkaline phosphatase activity, and bone formation were suppressed by implantation of the compacted BMG and that scarcely any vascularization occurred. Calcification, vascularization, and alkaline phosphatase activity were related and were indispensable for bone formation. In the control group, bone formation was observed at sites of high activity of alkaline phosphatase and well-developed vascularization. These results suggested that compacting of BMG suppressed vascularization, decreased calcification, and consequently reduced the induction of bone formation. |
| Databáze: | OpenAIRE |
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