Cellular pharmacology of P-ethoxy antisense oligonucleotides targeted to Bcl-2 in a follicular lymphoma cell line
| Autor: | Roberto Mercado-Hernández, Reyes Tamez-Guerra, Gabriel Lopez-Berestein, Ana M. Tari, Yolanda Gutiérrez-Puente, Richard J. Ford, Martha Santoyo-Stephano |
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| Rok vydání: | 2004 |
| Předmět: |
Cancer Research
Down-Regulation Biology chemistry.chemical_compound Drug Delivery Systems Downregulation and upregulation Tumor Cells Cultured Humans RNA Messenger Lymphoma Follicular Regulation of gene expression Messenger RNA Oligonucleotide RNA Hematology Oligonucleotides Antisense Molecular biology Gene Expression Regulation Neoplastic Ethyl Ethers Cell Transformation Neoplastic Oncology chemistry Proto-Oncogene Proteins c-bcl-2 Cell culture Gene Targeting Liposomes Growth inhibition Intracellular Cell Division |
| Zdroj: | Leukemialymphoma. 44(11) |
| ISSN: | 1042-8194 |
| Popis: | A P-ethoxy oligonucleotide (oligo), 20 bases long and specific for the translation initiation site of human Bcl-2 mRNA, was incorporated into liposomes to increase its intracellular delivery. This oligo selectively inhibited Bcl-2 protein expression and induced growth inhibition in t(14;18)-positive transformed follicular lymphoma (FL) cell lines. We studied the inhibitory effects of shorter liposomal P-ethoxy oligos (7, 9, 11 or 15 mer) in order to determine the activity of different oligo chain lengths targeted to the same Bcl-2 mRNA. At 12 microM, all the oligos inhibited the growth of a FL cell line. We compared the 7-mer oligo with the 20-mer oligo. The two oligos inhibited Bcl-2 protein expression similarly: 66% and 60% for the 7- and 20-mer, respectively. The uptake and retention of both oligos were also very similar. Our results indicate that the Bcl-2 inhibitory activity is maintained with P-ethoxy antisense oligos ranging from 7 to 20 bases. |
| Databáze: | OpenAIRE |
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