Thrombin generation: a comparison of assays using platelet-poor and -rich plasma and whole blood samples from healthy controls and patients with a history of venous thromboembolism
| Autor: | David Wynne Jones, K. A. Tappenden, G. Evans, Ian J. Mackie, M. J. Gallimore |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Adult
Blood Platelets Male medicine.medical_specialty Sensitivity and Specificity Statistics Nonparametric Tissue factor Plasma Thrombin Internal medicine Thromboembolism Blood plasma medicine Humans Platelet Blood coagulation test Whole blood business.industry Platelet-Rich Plasma Hematology Middle Aged Endocrinology Platelet-rich plasma Hemostasis Case-Control Studies Immunology Female Blood Coagulation Tests business Trypsin Inhibitors medicine.drug |
| ISSN: | 0007-1048 |
| Popis: | We have developed a whole blood thrombin generation (TG) assay whereby TG is initiated with a low-tissue factor concentration and monitored using a fluorogenic thrombin substrate. Significantly higher values were found in blood samples from 50 patients with a history of venous thromboembolism (VTE) compared with 31 healthy controls (HC), for peak height (P = 0.0034) and endogenous thrombin potential (ETP) (P = 0.0027). Results from 31 VTE patients and the 31 controls in the absence of corn trypsin inhibitor (CTI) showed significantly higher values in the VTE group for peak height (P = 0.0013) and ETP (P = 0.002). In the presence of CTI, significantly higher values were only seen in ETP (P = 0.024). No significant increases in TG were found using platelet poor (PPP) or -rich (PRP) plasma with or without CTI. The whole blood TG assay in the absence or presence of CTI showed a higher proportion (25/50 and 12/31, respectively) of raised peak height and/or ETP values than plasma assays (PPP 9/50 and 5/31 respectively and PRP 13/50 and 6/31, respectively). Our results show the whole blood TG assay is more sensitive for determining the increases in TG in patients with a history of VTE than PPP and PRP TG assays. |
| Databáze: | OpenAIRE |
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