Preventing contamination of PCR-based multiplex assays including the use of a dedicated biosafety cabinet
| Autor: | M. Drancourt, D. Raoult, Pierre-Yves Levy, A. Bouam, J.J. Vincent |
|---|---|
| Přispěvatelé: | Microbes évolution phylogénie et infections (MEPHI), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Male medicine.medical_specialty Biosafety cabinet Point-of-Care Systems [SDV]Life Sciences [q-bio] 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences 010608 biotechnology Internal medicine Cytology Multiplex polymerase chain reaction medicine False positive paradox Prevalence Humans Multiplex Meningitis Retrospective Studies 0303 health sciences Bacteria 030306 microbiology business.industry Contamination Containment of Biohazards medicine.disease 3. Good health 13. Climate action Viruses Encephalitis Female business Laboratories Multiplex Polymerase Chain Reaction |
| Zdroj: | LETTERS IN APPLIED MICROBIOLOGY LETTERS IN APPLIED MICROBIOLOGY, 2021, 72 (1), pp.98-103. ⟨10.1111/lam.13375⟩ |
| Popis: | We retrospectively investigated cases of false-positive diagnoses using the BIOFIRE (R) FilmArray (R) meningitis/encephalitis (ME) panel to measure the impact of using a dedicated biosafety cabinet combined with preventive measures to reduce the prevalence of false-positive diagnoses due to pre-analytical in-laboratory contamination. False-positive results were identified by reviewing clinical data, biological parameters and cytology results of cerebrospinal fluid (CSF) samples showing discrepant results between the FilmArray ME panel and routine PCR assays. A total of 327 CSF were analysed over 16 weeks in point-of-care (POC) A and B, over two 8-week periods, periods 1 and 2. The analysis yielded 30 (9 center dot 17%) detection of at least one pathogen including 21/30 (70%) viruses and 9/30 (30%) bacteria. During period 1, POC-A and POC-B manipulated CSF under a non-dedicated hood featuring laminar flow, whereas during period 2, CSFs were manipulated under a dedicated biosafety cabinet without any airflow in POC-A. During period 1, false positives were detected in 3/114 CSF (2 center dot 63%) in POC-A and 1/36 (2 center dot 77%) in POC-B (P = 0 center dot 97); during period 2, false positives were detected in 0/139 CSF (0%) in POC-A and 1/38 (2 center dot 63%) in POC-B (P = 0 center dot 23). All false positives were bacterial. The use of a dedicated cabinet without ventilation along with preventive measures during period 2 in POC-A significantly reduced the number of false-positive results (P = 0 center dot 05). Preventive measures described in this study can mitigate false positives when using PCR-based multiplex assays such as the BIOFIRE FilmArray ME Panel for the diagnosis of meningitis and other infectious diseases. |
| Databáze: | OpenAIRE |
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