Effect of cadmium on some enzyme activities in first-trimester human placentae
| Autor: | Joseph M. Brandes, Jacob Urbach, Shmuel Yannai, William Y. Boadi |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Placenta
Dehydrogenase Glucosephosphate Dehydrogenase Reductase Catechol O-Methyltransferase Toxicology Incubation period Andrology chemistry.chemical_compound Pregnancy Culture Techniques NAD(P)H Dehydrogenase (Quinone) medicine Humans Glucose-6-phosphate dehydrogenase Incubation chemistry.chemical_classification General Medicine Pregnancy Trimester First Enzyme medicine.anatomical_structure chemistry Biochemistry Toxicity Female Aryl Hydrocarbon Hydroxylases Cadmium |
| Zdroj: | Toxicology Letters. 60:155-164 |
| ISSN: | 0378-4274 |
| Popis: | Explants from first-trimester placentae obtained from non-smoking women were incubated with doses of 0, 0.75, 1.5, 3, 6 and 12 micrograms of cadmium (Cd) as CdCl2 for 6 or 24 h. At the end of the incubation period, the activities of placental aryl hydrocarbon hydroxylase (AHH) (a phase I enzyme), quinone reductase (QR) and catecholamine-O-methyltransferase (COMT) (both phase II enzymes) and glucose-6-phosphate dehydrogenase (G-6-PD) were determined. Cd at low dose levels increased significantly the activities of placental phases I and II enzymes in a time- and dose-dependent manner. Of the first 3 enzymes, only AHH showed a biphasic response for the two time periods, with the activities of QR and COMT continually increasing at all the dose levels tested for the two incubation periods. However, the G-6-PD activity was inhibited at all the dose levels of Cd, the effect being very drastic after exposure to 0.75 ppm Cd for both incubation periods. |
| Databáze: | OpenAIRE |
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