Patterns of gene expression in pig adipose tissue: Insulin-like growth factor system proteins, neuropeptide Y (NPY), NPY receptors, neurotrophic factors and other secreted factors
| Autor: | Gary J. Hausman, C R Barb, R. G. Dean |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Swine medicine.medical_treatment Adipose tissue macrophages Subcutaneous Fat Adipose tissue Connective tissue Ciliary neurotrophic factor Insulin-like growth factor Endocrinology Food Animals Somatomedins Neurotrophic factors Internal medicine Gene expression medicine Animals Neuropeptide Y Nerve Growth Factors Oligonucleotide Array Sequence Analysis biology Gene Expression Profiling Leptin Age Factors Receptors Neuropeptide Y Insulin-Like Growth Factor Binding Proteins medicine.anatomical_structure biology.protein Intercellular Signaling Peptides and Proteins Animal Science and Zoology Growth and Development |
| Zdroj: | Domestic Animal Endocrinology. 35:24-34 |
| ISSN: | 0739-7240 |
| DOI: | 10.1016/j.domaniend.2008.01.004 |
| Popis: | Although cDNA microarray studies have examined gene expression in human and rodent adipose tissue, only one microarray study of adipose tissue from growing pigs has been reported. Total RNA was collected at slaughter from outer subcutaneous adipose tissue (OSQ) and middle subcutaneous adipose tissue (MSQ) from gilts at 90, 150, and 210d ( n =5age −1 ). Dye labeled cDNA probes were hybridized to custom porcine microarrays (70-mer oligonucleotides). Gene expression of insulin-like growth factor binding proteins (IGFBPs), hormones, growth factors, neuropeptide Y (NPY) receptors (NPYRs) and other receptors in OSQ and MSQ changed little with age in growing pigs. Distinct patterns of relative gene expression were evident within NPYR and IGFBP family members in adipose tissue from growing pigs. Relative gene expression levels of NPY2R, NPY4R and angiopoietin 2 (ANG-2) distinguished OSQ and MSQ depots in growing pigs. We demonstrated, for the first time, the expression of IGFBP-7, IGFBP-5, NPY1R, NPY2R, NPY, connective tissue growth factor (CTGF), brain-derived neurotrophic factor (BDNF) and ciliary neurotrophic factor (CNTF) genes in pig adipose tissue with microarray and RT-PCR assays. Furthermore, adipose tissue CTGF gene expression was upregulated while NPY and NPY2R gene expression were significantly down regulated by age. These studies demonstrate that expression of neuropeptides and neurotrophic factors in pig adipose tissue may be involved in regulation of leptin secretion. Many other regulatory factors were not influenced by age in growing pigs but may be influenced by location or depot. |
| Databáze: | OpenAIRE |
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