Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid: Comparative Characterization on the Nanoscale
| Autor: | Kristin J. Flores, Adam K. Wanekaya, Christopher M Reynolds, Lisa Cillessen, Michael Fisher, Stephanie Barber, Tiffany Severs, Robert K. Delong, Ashley Schaeffer, Kartik Ghosh, John Black |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Gel electrophoresis
Materials science Article Subject biology 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Protamine 0104 chemical sciences chemistry.chemical_compound chemistry Biochemistry Colloidal gold Drug delivery lcsh:Technology (General) Nucleic acid biology.protein Nanobiotechnology lcsh:T1-995 General Materials Science 0210 nano-technology Nanoconjugates DNA |
| Zdroj: | Journal of Nanotechnology, Vol 2012 (2012) |
| ISSN: | 1687-9511 1687-9503 |
| Popis: | DNA and RNA micro- and nanoparticles are increasingly being used for gene and siRNA drug delivery and a variety of other applications in bionanotechnology. On the nanoscale, these entities represent unique challenges from a physicochemical characterization perspective. Here, nucleic acid conjugates with protamine and gold nanoparticles (GNP) were characterized comparatively in the nanorange of concentration by UV/Vis NanoDrop spectroscopy, fluorimetry, and gel electrophoresis. Given the intense interest in splice-site switching oligomers (SSOs), we utilized a human tumor cell culture system (HeLa pLuc-705), in which SSO-directed splicing repair upregulates luciferase expression, in order to investigate bioactivity of the bionanoconjugates. Process parameters important for bioactivity were investigated, and the bimolecular nanoconjugates were confirmed by shifts in the dynamic laser light scatter (DLLS), UV/Vis spectrum, gel electrophoresis, or sedimentation pattern. The data presented herein may be useful in the future development of pharmaceutical and biotechnology formulations, processes, and analyses concerning protein, DNA, or RNA bionanoconjugates. |
| Databáze: | OpenAIRE |
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