Production and characterization of antibodies against fumigaclavine A

Autor:	Yvonne Ackermann, Madeleine Groß, Hadri Latif, Ewald Usleber, V. Curtui
Rok vydání:	2009
Předmět:	Antiserum Detection limit Chromatography biology medicine.diagnostic_test Chemistry Toxicology Microbiology Standard curve Lysergic acid chemistry.chemical_compound Polyclonal antibodies Immunoassay biology.protein medicine Antibody Biotechnology Conjugate
Zdroj:	Mycotoxin Research. 25:159-164
ISSN:	1867-1632 0178-7888
DOI:	10.1007/s12550-009-0024-0
Popis:	Polyclonal antibodies against fumigaclavine A (FuA) were obtained from rabbits after immunization with a FuA-keyhole limpet hemocyanine conjugate prepared by formaldehyde condensation. Using these antibodies and a FuA-bovine serum albumine conjugate, a competitive indirect enzyme immunoassay (EIA) was established. The antiserum obtained from one rabbit enabled highly sensitive detection of FuA, with an IC50 level and detection limit of the standard curve of 3.3 ng/ml and approx. 1 ng/ml, respectively. The EIA was very specific for FuA, with 1.3% cross-reactivity with FuB. Several other lysergic acid derivatives (ergonovine, ergotamine, alpha-ergocryptine) were tested but did not cross-react in the FuA EIA. This is the first description of antibodies against FuA and the first development of an EIA for FuA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96348d727e9d532103553274b62fb9be https://doi.org/10.1007/s12550-009-0024-0 Zobrazit plný text záznamu Full text from SpringerLink