Micromanipulation of cryopreserved embryos and cryopreservation of micromanipulated embryos in PGD
| Autor: | Eleonora Porcu, A.S. Ricco, Raffaella Fabbri, C. Lagalla, Patrizia Ciotti, A. Forabosco |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
animal structures
Cell Survival Biopsy Biology Preimplantation genetic diagnosis Biochemistry Chromosomes Cryopreservation Specimen Handling Andrology Endocrinology Pregnancy medicine Humans Blastocyst Molecular Biology In Situ Hybridization Fluorescence Preimplantation Diagnosis Cell survival medicine.diagnostic_test Fish analysis Embryo Anatomy medicine.anatomical_structure embryonic structures Fish Female |
| Zdroj: | Molecular and Cellular Endocrinology. 169:63-67 |
| ISSN: | 0303-7207 |
| Popis: | The possibility to employ cryopreservation in Preimplantation Genetic Diagnosis (PGD) should enlarge the opportunities for research and clinical activity. For these purposes, we tried three kinds of approaches on human abnormal embryos: (1) cryopreservation of biopsied embryos; (2) biopsy of thawed embryos; and (3) biopsy of embryos derived from thawed oocytes. Our preliminary results show that: (1) biopsy of thawed embryos is feasible and FISH analysis is possible on both survived and lysed cells; (2) Optimization of freezing/thawing procedures are necessary to obtain better survival rate after thawing of biopsied embryos; (3) Biopsy and FISH are feasible on embryos derived from thawed oocytes and they could be a good way to study the chromosomal arrangement of these poorly investigated embryos. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect