| Autor: |
A. G. Fredrickson, Friedrich Srienc, Jia Jer Liou |
| Rok vydání: |
1998 |
| Předmět: |
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| Zdroj: |
Biotechnology Progress. 14:450-456 |
| ISSN: |
8756-7938 |
| DOI: |
10.1021/bp980019u |
| Popis: |
A selection synchronization technique based on ingestion of tantalum particles has been applied to obtain synchronized cultures of the filter feeding ciliate Tetrahymena pyriformis. Cell concentrations and cell volume distributions of synchronized cell populations have been monitored for more than four average generation times. A simple curve-fitting method has been used to decompose the cell volume distributions of a synchronous population into two populations representing cells before and after cell division. In this way, the time course of the growth of an initially synchronous culture is decomposed into the growth of successive generations. The data indicate that at any given time only two generations of cells are present in significant numbers. The measured cell volume distributions show that T. pyriformis has a complicated growth pattern during the cell cycle. Newborn T. pyriformis cells do not grow significantly at the beginning of the cell cycle. After the nongrowing stage, cells start growing in a possibly exponential rate before cells enter into a second nongrowing stage. The second nongrowing stage lasts until cell division. The presented data demonstrate that growing cell populations can be viewed as the composite of cells belonging to different generations. This concept has important implications for solving corpuscular models of cell growth. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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