Matrine inhibiting pancreatic cells epithelial-mesenchymal transition and invasion through ROS/NF-κB/MMPs pathway
| Autor: | Wen Xin, Minna Huang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Epithelial-Mesenchymal Transition Cell Survival Apoptosis General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound Alkaloids 0302 clinical medicine Gentamicin protection assay Matrine Cell Line Tumor Pancreatic cancer medicine Humans Epithelial–mesenchymal transition General Pharmacology Toxicology and Pharmaceutics Matrines Wound Healing Dose-Response Relationship Drug Chemistry NF-kappa B NF-κB General Medicine medicine.disease Antineoplastic Agents Phytogenic Reactive Nitrogen Species Matrix Metalloproteinases IκBα 030104 developmental biology 030220 oncology & carcinogenesis Cancer cell Cancer research Quinolizines Signal Transduction |
| Zdroj: | Life Sciences. 192:55-61 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/j.lfs.2017.11.024 |
| Popis: | Aims Matrine has demonstrated an exclusive anti-tumor effect, including inhibiting cancer cells proliferation and inducing cancer cells apoptosis and autophagy. Whether it can inhibit cancer cells invasion is remain obscure. Main methods The Panc-1 cells were cultured with matrine, NAC and methanol, wound healing assay and transwell invasion assay were applied to detect the migration and invasion ability. The expression of MMP-2 and MMP-9 were assessed, as well as the Epithelial-Mesenchymal Transition marker. Further detect the expression of pP65, total P65, pIκBα, total IκBα, MMP-2, MMP-9 and Panc-1 cells migration and invasion ability to detect whether NF-κB signaling pathway is involved. Key findings In matrine treated group, the expression of E-cadherin was up-regulated while N-cadherin, Vimentin was down-regulated. In addition, wound healing assay and transwell invasion assay showed that the cells treated with matrine expressed weaker migration and invasion ability, and MMP-2 and MMP-9 was down-regulated in matrine treated group. Further research reveals that the effect of Matrine could decreased the level of intracellular ROS. Furthermore, pP65, pIκBα level was down-regulated in the matrine and NAC group when compared to control group. The panc-1 cells showed less migration and invasion ability, as well as lower MMP-2 and MMP-9 expression in the group treated with NF-κBI along with H2O2 when compared with treated with H2O2 only. Significance Matrine inhibit pancreatic cancer cells migration and invasion through ROS/NF-κB/MMPs pathway, further validate the anticancer effect of matrine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect