Autophagic event and metabolomic disorders unveil cellular toxicity of environmental microplastics on marine polychaete Hediste diversicolor

Autor: Omayma Missawi, Massimo Venditti, Tiziana Cappello, Nesrine Zitouni, Giuseppe DE. Marco, Iteb Boughattas, Noureddine Bousserrhine, Sabrina Belbekhouche, Sergio Minucci, Maria Maisano, Mohamed Banni
Přispěvatelé: Institut de Chimie et des Matériaux Paris-Est (ICMPE), Institut de Chimie du CNRS (INC)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Missawi, Omayma, Venditti, Massimo, Cappello, Tiziana, Zitouni, Nesrine, Marco, Giuseppe DE., Boughattas, Iteb, Bousserrhine, Noureddine, Belbekhouche, Sabrina, Minucci, Sergio, Maisano, Maria, Banni, Mohamed
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Environmental Pollution
Environmental Pollution, Elsevier, 2022, 302 (8), pp.119106. ⟨10.1016/j.envpol.2022.119106⟩
ISSN: 0269-7491
1873-6424
DOI: 10.1016/j.envpol.2022.119106⟩
Popis: Although the hazards of microplastics (MPs) have been quite well explored, the aberrant metabolism and the involvement of the autophagy pathway as an adverse response to environmental MPs in benthic organisms are still unclear. The present work aims to assess the impact of different environmental MPs collected from the south coast of the Mediterranean Sea, composed by polyethylene (PE), polyethylene vinyl acetate (PEVA), low-density polyethylene (LDPE), high-density polyethylene (HDPE), polypropylene (PP) and polyamide (PA) on the metabolome and proteome of the marine polychaete Hediste diversicolor. As a result, all the microplastic types were detected with Raman microspectroscopy in polychaetes tissues, causing cytoskeleton damage and induced autophagy pathway manifested by immunohistochemical labeling of specific targeted proteins, through Tubulin (Tub), Microtubule-associated protein light chain 3 (LC3), and p62 (also named Sequestosome 1). Metabolomics was conducted to further investigate the metabolic alterations induced by the environmental MPs-mixture in polychaetes. A total of 28 metabolites were differentially expressed between control and MPs-treated polychaetes, which showed elevated levels of amino acids, glucose, ATP/ADP, osmolytes, glutathione, choline and phosphocholine, and reduced concentration of aspartate. These novel findings extend our understanding given the toxicity of environmental microplastics and unravel their underlying mechanisms.
Databáze: OpenAIRE
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