Allo-HLA Cross-Reactivities of Cytomegalovirus-, Influenza-, and Varicella Zoster Virus-Specific Memory T Cells Are Shared by Different Healthy Individuals
| Autor: | S. L. Yong, Xiaoqian Zhang, M. E.I. Franke-van Dijk, P. P. M. C. van Miert, E. M.W. van der Meer-Prins, R. J. M. Ten Berge, Kirstin M. Heutinck, Jacqueline D.H. Anholts, Frans H.J. Claas, Dave L. Roelen, H. van den Heuvel |
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| Přispěvatelé: | Other departments, AII - Infectious diseases, AII - Amsterdam institute for Infection and Immunity, Nephrology |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Herpesvirus 3 Human medicine.medical_treatment Cytomegalovirus Immunogenetics Human leukocyte antigen CD8-Positive T-Lymphocytes Cross Reactions Biology medicine.disease_cause 03 medical and health sciences HLA Antigens Influenza Human medicine Humans Immunology and Allergy Pharmacology (medical) Transplantation T-cell receptor Varicella zoster virus Orthomyxoviridae Virology Healthy Volunteers Histocompatibility 030104 developmental biology Cytokine Varicella Zoster Virus Infection Cytomegalovirus Infections Immunology Immunologic Memory CD8 |
| Zdroj: | American journal of transplantation, 17(8), 2033-2044. Wiley-Blackwell American Journal of Transplantation, 17(8), 2033-2044. WILEY American Journal of Transplantation, 17(8), 2033-2044 |
| ISSN: | 1600-6135 |
| DOI: | 10.1111/ajt.14279 |
| Popis: | Virus-specific T cells can recognize allogeneic HLA (allo-HLA) through TCR cross-reactivity. The allospecificity often differs by individual (private cross-reactivity) but also can be shared by multiple individuals (public cross-reactivity); however, only a few examples of the latter have been described. Because these could facilitate alloreactivity prediction in transplantation, we aimed to identify novel public cross-reactivities of human virus-specific CD8(+) T cells directed against allo-HLA by assessing their reactivity in mixed-lymphocyte reactions. Further characterization was done by studying TCR usage with primer-based DNA sequencing, cytokine production with ELISAs, and cytotoxicity with 51 chromium-release assays. We identified three novel public allo-HLA cross-reactivities of human virus-specific CD8(+) T cells. CMV B35/IPS CD8(+) T cells cross-reacted with HLA-B51 and/or HLA-B58/B57 (23% of tetramer-positive individuals), FLU A2/GIL (influenza IMP[58-66] HLA-A* 02: 01/GILGFVFTL) CD8(+) T cells with HLA-B38 (90% of tetramer-positive individuals), and VZV A2/ALW (varicella zoster virus IE62[593-601] HLA-A*02:01/ALWALPHAA) CD8(+) T cells with HLA-B55 (two unrelated individuals). Cross-reactivity was tested against different cell types including endothelial and epithelial cells. All cross-reactive T cells expressed a memory phenotype, emphasizing the importance for transplantation. We conclude that public allo-HLA cross-reactivity of virus-specific memory T cells is not uncommon and may create novel opportunities for alloreactivity prediction and risk estimation in transplantation. |
| Databáze: | OpenAIRE |
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