Leucine alleviates dexamethasone-induced suppression of muscle protein synthesis via synergy involvement of mTOR and AMPK pathways
| Autor: | Hong Chao Jiao, Jing Peng Zhao, Z. G. Song, Ru Xia Wang, Hai Lin, Xiao Juan Wang, Xin Yang |
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| Rok vydání: | 2016 |
| Předmět: |
AMPK
0301 basic medicine endocrine system protein synthesis Biophysics S7 Muscle Proteins P70-S6 Kinase 1 AMP-Activated Protein Kinases Mitogen-activated protein kinase kinase S43 Biochemistry mTORC2 Dexamethasone Cell Line Myoblasts Mice 03 medical and health sciences Leucine polycyclic compounds Animals ASK1 c-Raf Phosphorylation Glucocorticoids Molecular Biology Protein kinase B Original Paper biology Akt/PKB signaling pathway TOR Serine-Threonine Kinases S13 Cell Biology Original Papers S10 muscle cell Cell biology 030104 developmental biology Protein Biosynthesis mTOR biology.protein glucocorticoid hormones hormone substitutes and hormone antagonists Signal Transduction RHEB |
| Zdroj: | Bioscience Reports |
| ISSN: | 1573-4935 0144-8463 |
| Popis: | Both mTOR and AMPK pathways are involved in the DEX-induced suppression of protein synthesis in muscle cells. Leucine supplementation relieves DEX-induced inhibition on protein synthesis by evoking mTOR and suppressing AMPK pathway. Glucocorticoids (GCs) are negative muscle protein regulators that contribute to the whole-body catabolic state during stress. Mammalian target of rapamycin (mTOR)-signalling pathway, which acts as a central regulator of protein metabolism, can be activated by branched-chain amino acids (BCAA). In the present study, the effect of leucine on the suppression of protein synthesis induced by GCs and the pathway involved were investigated. In vitro experiments were conducted using cultured C2C12 myoblasts to study the effect of GCs on protein synthesis, and the involvement of mTOR pathway was investigated as well. After exposure to dexamethasone (DEX, 100 μmol/l) for 24 h, protein synthesis in muscle cells was significantly suppressed (P0.05) but was increased by leucine supplementation at a dose of 5 mmol/l (P |
| Databáze: | OpenAIRE |
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