Blot hybridisation analysis of genomic DNA
| Autor: | S Vandenplas, I Wiid, G Wallis, K Brebner, M Ricketts, A J Bester, Anne F. Grobler-Rabie, C G Mathew, C Boyd |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Electrophoresis
Agar Gel Genetics DNA Recombinant Nucleic Acid Hybridization DNA DNA Restriction Enzymes Biology DNA sequencing Restriction fragment Blot genomic DNA chemistry.chemical_compound Nucleic acid thermodynamics Restriction enzyme chemistry biology.protein Autoradiography Humans Restriction fragment length polymorphism Molecular Biology Genetics (clinical) Research Article |
| Zdroj: | Journal of Medical Genetics. 21:164-172 |
| ISSN: | 1468-6244 |
| DOI: | 10.1136/jmg.21.3.164 |
| Popis: | Restriction endonuclease analysis of specific gene sequences is proving to be a valuable technique for characterisation and diagnosis of inherited disorders. This paper describes detailed protocols for isolation, restriction, and blot hybridisation of genomic DNA. Problems and alternatives in the procedure are discussed and a troubleshooting guide has been provided to help rectify faults. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|