Expression of mdr1 and mrp in the normal B‐cell homologue of B‐cell chronic lymphocytic leukaemia
| Autor: | Phillip Kantharidis, S. T. Chou, D. Tzelepis, I. Bertoncello, Dominic M. Wall, John D. Parkin, John Zalcberg, Sam El-Osta |
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| Rok vydání: | 1997 |
| Předmět: |
ATP Binding Cassette Transporter
Subfamily B Chronic lymphocytic leukemia Population B-Lymphocyte Subsets Biology CD19 Immunophenotyping T-Lymphocyte Subsets immune system diseases hemic and lymphatic diseases Gene expression Leukemia B-Cell Tumor Cells Cultured medicine Humans ATP Binding Cassette Transporter Subfamily B Member 1 RNA Messenger education neoplasms B cell education.field_of_study Hematology medicine.disease Phenotype Molecular biology Drug Resistance Multiple Multiple drug resistance medicine.anatomical_structure Drug Resistance Neoplasm Immunology biology.protein ATP-Binding Cassette Transporters CD5 |
| Zdroj: | British Journal of Haematology. 96:697-707 |
| ISSN: | 1365-2141 0007-1048 |
| DOI: | 10.1046/j.1365-2141.1997.d01-2090.x |
| Popis: | B-cell chronic lymphocytic leukaemia (CLL) cells commonly express the multidrug resistance phenotype. The aim of this study was to establish whether the normal homologue in B-cell ontogeny of B-CLL also expressed the multidrug resistance (mdr) phenotype. Human tonsillar lymphocytes were sorted to yield two B-cell subsets based on the expression of CD19, CD5 and CD10. The normal homologue was represented by a population of B cells that was CD19 positive, CD10 negative and weakly expressed CD5. Based upon functional analysis and the detection of mdr1 mRNA by semi-quantitative PCR, these cells expressed the mdr phenotype. In contrast, functional multidrug resistance could not be demonstrated in CD19-positive CD10-positive cells with strong expression of CD5, nor could mdr1 mRNA be found in these cells. MRP was variably expressed in both B-cell subsets with no discernable differences in the pattern of expression. We conclude that normal B cells with a phenotype resembling that of B-CLL cells express the multidrug resistance phenotype. |
| Databáze: | OpenAIRE |
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