The use of α-(N-γ-dl-glutamyl)-aminonitriles for the colorimetric determination of a specific peptidase in blood serum
| Autor: | Marian Orłowski, Apolinary Szewczuk |
|---|---|
| Rok vydání: | 1960 |
| Předmět: |
Serum
chemistry.chemical_classification Cyanides Chromatography Hydrolases Biochemistry (medical) Clinical Biochemistry Substrate (chemistry) General Medicine Urine Biology Biochemistry Enzyme assay Hydrolysis Enzyme Blood serum chemistry biology.protein Colorimetry Amino Acids Incubation Peptide Hydrolases |
| Zdroj: | Clinica Chimica Acta. 5:680-688 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(60)90009-7 |
| Popis: | The occurrence of a specific peptidase in human serum is described. The enzyme catalyses the hydrolysis of α-(N-γ- dl -glutamyl)-aminopropionitrile as well as of γ-glutamyl derivatives of other α-aminonitriles. A method is described for assay of the enzyme activity, based on the colorimetric determination of α-aminopropionitrile liberated during the enzymatic incubation ; as little as 0.2 μg of α-aminopropionitrile can be estimated. Some properties of the enzyme were investigated and its specificity is discussed. The activity in sera of normal human subjects was 10.9 units (1 unit = 1 μmole α-aminopropionitrile liberated from the substrate per 100 ml serum after a 2-h incubation at pH 8.6 and 37°). Changes in the activity of the peptidase were found in some pathological conditions. Besides human serum, the enzyme activity was found in sera of many animals, as well as in human urine and pathological exudations. No activity was found in human red blood cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|