Comparative study of the plasma globulin level, CD21− B-cell counts and FOXP3 mRNA expression level in CD4+ T-cells for different clinical stages of feline immunodeficiency virus infected cats
| Autor: | Tomomi Takano, Ryoichi Satoh, Bunpei Nagasaki, Akari Shibao, Hisao Yoshioka, Shinobu Hosoya, Tsutomu Hohdatsu |
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| Rok vydání: | 2012 |
| Předmět: |
CD4-Positive T-Lymphocytes
Feline immunodeficiency virus Regulatory T cell Immunodeficiency Virus Feline Biology Cat Diseases T-Lymphocytes Regulatory Feline Acquired Immunodeficiency Syndrome Hypergammaglobulinemia medicine Animals Lymphocyte Count RNA Messenger B cell B-Lymphocytes Arc (protein) CATS General Veterinary FOXP3 Forkhead Transcription Factors medicine.disease biology.organism_classification Molecular biology medicine.anatomical_structure Case-Control Studies Immunology Cats Disease Progression Lentivirus Infections Receptors Complement 3d Serum Globulins Asymptomatic carrier |
| Zdroj: | Research in Veterinary Science. 92:157-161 |
| ISSN: | 0034-5288 |
| Popis: | Feline immunodeficiency virus (FIV) infection leads to hypergammaglobulinemia through mechanisms that remain poorly understood. We investigated changes in plasma globulin level, B cells, and T cells with progression of the clinical stage of FIV-infected cats. We classified FIV-infected cats into the stage of Asymptomatic carrier (AC) and AIDS-related complex (ARC) based on the clinical symptoms, and measured the plasma globulin level, the CD4+ T-cell counts, and analyzed surface markers of B cells. We investigated the relationship between the plasma globulin level and regulatory T cells (Tregs) using the Forkhead box P3 (FOXP3) mRNA expression level. In FIV-infected cats, the plasma globulin level and the surface immunoglobulin (sIg)+ CD21− B-cell counts were increased, whereas the CD4+ T-cell counts were decreased compared with specific-pathogen free (SPF) cats. The mRNA expression of Blimp-1 (master gene of plasma cells) was increased in peripheral blood, and the FOXP3 mRNA expression level was decreased in CD4+ T-cells. These immunological changes were marked in the ARC stage. These data indicate that the decrease of Tregs and the increase of plasma cells lead to hypergammaglobulinemia. |
| Databáze: | OpenAIRE |
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