BIN4, a Novel Component of the Plant DNA Topoisomerase VI Complex, Is Required for Endoreduplication in Arabidopsis[W][OA]
| Autor: | Keiko Sugimoto-Shirasu, Christian Breuer, Christopher E. West, Keith Roberts, Yunde Zhao, Yoshitaka Azumi, Nicola Stacey, Anthony Maxwell, Hirokazu Tsukaya, Joanne Chory |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
G2 Phase
DNA damage Mutant Molecular Sequence Data Arabidopsis Mitosis Cell Cycle Proteins Plant Science Ataxia Telangiectasia Mutated Proteins Protein Serine-Threonine Kinases Genes Plant Plant Epidermis Steroids Heterocyclic Gene Expression Regulation Plant Brassinosteroids Arabidopsis thaliana Endoreduplication Amino Acid Sequence Cyclin B1 Gene Research Articles Cell Proliferation Cell Size Genetics Cell Nucleus Ploidies biology Arabidopsis Proteins Tumor Suppressor Proteins Cell Biology DNA Organ Size biology.organism_classification Nuclear DNA Cell biology DNA-Binding Proteins Phenotype DNA Topoisomerases Type I Mutation Colchicine Cholestanols DNA Damage |
| Popis: | How plant organs grow to reach their final size is an important but largely unanswered question. Here, we describe an Arabidopsis thaliana mutant, brassinosteroid-insensitive4 (bin4), in which the growth of various organs is dramatically reduced. Small organ size in bin4 is primarily caused by reduced cell expansion associated with defects in increasing ploidy by endoreduplication. Raising nuclear DNA content in bin4 by colchicine-induced polyploidization partially rescues the cell and organ size phenotype, indicating that BIN4 is directly and specifically required for endoreduplication rather than for subsequent cell expansion. BIN4 encodes a plant-specific, DNA binding protein that acts as a component of the plant DNA topoisomerase VI complex. Loss of BIN4 triggers an ATM- and ATR-dependent DNA damage response in postmitotic cells, and this response coincides with the upregulation of the cyclin B1;1 gene in the same cell types, suggesting a functional link between DNA damage response and endocycle control. |
| Databáze: | OpenAIRE |
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