Development and validation of analytical method for quantitation of Emtricitabine, Tenofovir, Efavirenz based on HPLC
| Autor: | Arun Ramaswamy, Anton Smith Arul Gnana Dhas |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Efavirenz
Tenofovir Chemistry(all) General Chemical Engineering 02 engineering and technology 010402 general chemistry Emtricitabine 01 natural sciences High-performance liquid chromatography lcsh:Chemistry chemistry.chemical_compound medicine Hplc method HPLC-UV Chromatography Antiretrovirals General Chemistry 021001 nanoscience & nanotechnology Emtricitabine/Tenofovir 0104 chemical sciences lcsh:QD1-999 chemistry Injection volume Chemical Engineering(all) Uv detection 0210 nano-technology medicine.drug |
| Zdroj: | Arabian Journal of Chemistry, Vol 11, Iss 2, Pp 275-281 (2018) |
| ISSN: | 1878-5352 |
| DOI: | 10.1016/j.arabjc.2014.08.007 |
| Popis: | This paper describes the development and validation of a HPLC method for the quantitation of Emtricitabine, Tenofovir, and Efavirenz in pure form and pharmaceutical formulations. The Zorbax SB CN, (250 × 4.6 mm, 5 μm) column was used. UV detection was performed at 260 nm. The mobile phase consisted of methanol (A) and buffer at pH 4.5(B) using the gradient: 0–10 min (90% B), 10–22 min (35% B), and 22–25 min (90% B). The flow rate was 1.5 ml/min in ambient temperature. The injection volume of sample was 20 μl. The method showed to be linear (r2 > 0.999), precise (RSD |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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