HIV P24 antigen among HIV antibody seronegative blood donors in Osogbo Osun State, South Western Nigeria
| Autor: | Emmanuel Kehinde Fadeni, Olusola Anuoluwapo Akanbi, Olusolabomi J. Adefioye, Adeolu Sunday Oluremi, OA Olowe, R. A. Olowe, V. O. Mabayoje, Oluyinka Oladele Opaleye |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine Adolescent 030106 microbiology HIV Core Protein p24 Nigeria Blood Donors Enzyme-Linked Immunosorbent Assay HIV Infections Window period HIV Antibodies medicine.disease_cause Microbiology Young Adult 03 medical and health sciences 0302 clinical medicine Antigen HIV Seronegativity medicine Humans 030212 general & internal medicine Child Demography Hepatitis B virus biology Transmission (medicine) business.industry Public Health Environmental and Occupational Health virus diseases Original Articles General Medicine Hepatitis C Middle Aged medicine.disease Virology Infectious Diseases HIV-2 HIV p24 Antigen Immunology HIV-1 biology.protein Female Parasitology Antibody business |
| Zdroj: | Pathogens and Global Health. 110:205-208 |
| ISSN: | 2047-7732 2047-7724 |
| Popis: | Efforts to curb the spread of HIV transmission through transfusion of blood and its products is still a problem because of challenge in countries using antibody-based rapid methods to detect infection during window period. Transmission of HIV through infected blood and its products accounts for approximately 10% in African region.This study analyzed true negativity of HIV infection in blood donors screened by ELISA test based on p24 core antigen detection. Four hundred and eighty (480) blood donors initially negative for HIV antibody by rapid screening kit, Determine™ HIV-1/2 (Abbott Laboratory, IL, USA) and re-screened with Immuno Comb® II HIV 1 and 2 (Bispot kit PBS Organics and Israel 2005). The samples were further tested for the presence of HIV antibody and p24 HIV core antigen using ELISA kits (Genscreen TM ULTRA HIV Ag-Ab) following manufacturer's instructions. All donors initially tested negative for Hepatitis B virus, Hepatitis C virus.Two (0.42%) of 480 blood donors tested positive for the p24 HIV core antigen. The two positive donors for the p24 antigen had multiple sexual partners and recent sexually transmitted infections.The association of the HIV p24 antigen with blood donation was highly significant (p = 0.000) and pose a great risk to recipients if screening of blood donor is only carried out by HIV antibody detection. |
| Databáze: | OpenAIRE |
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