Polyethyleneimine grafted with pluronic P85 enhances Ku86 antisense delivery and the ionizing radiation treatment efficacy in vivo
| Autor: | T Y K Chow, Serguei V. Vinogradov, B P Monia, A I Belenkov, V Y Alakhov, Lawrence Panasci, Alexander V. Kabanov |
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| Rok vydání: | 2004 |
| Předmět: |
Genetic enhancement
Transplantation Heterologous Heterologous Gene Expression Biology Gene delivery Transfection Mice In vivo Cell Line Tumor Neoplasms Genetics Animals Humans Polyethyleneimine Molecular Biology Ku Autoantigen Mice Inbred BALB C Oligonucleotide Antigens Nuclear Genetic Therapy Oligonucleotides Antisense Molecular biology Transplantation DNA-Binding Proteins Cell culture Molecular Medicine Poloxalene Female Neoplasm Transplantation |
| Zdroj: | Gene therapy. 11(22) |
| ISSN: | 0969-7128 |
| Popis: | In an effort to improve the efficacy of antisense delivery, we evaluated polyethyleneimine (PEI, 2 kDa) alone or grafted with nonionic amphiphilic block copolymer Pluronic (P85) as a carrier for Ku86 antisense oligonucleotide (ASO) delivery. Ku86 is an abundant nuclear protein that plays an important role in nonhomologous DNA end joining and has implications in tumorigenesis and acquired drug resistance. Transfection of adherent and suspension cell lines with Ku86 ASOs complexed with P85-g-PEI (2 kDa) conjugates was associated with a specific decrease in Ku86 mRNA levels (EC50 |
| Databáze: | OpenAIRE |
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