Osteoblast adherence and resorption activity of isolated osteoclasts on calcium sulphate hemihydrate
| Autor: | P. Collin, M. Sidqui, N. Forest, C. Vitte |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Calcitonin
Male musculoskeletal diseases Neutral red Acid Phosphatase Biophysics Osteoclasts chemistry.chemical_element Bioengineering Calcium Calcium Sulfate Rats Sprague-Dawley Biomaterials Mice chemistry.chemical_compound Osteoclast Cell Adhesion medicine Animals Femur Bone Resorption Carbonic Anhydrase Inhibitors Tartrates Cells Cultured Osteoblasts biology Acid phosphatase Osteoblast Alkaline Phosphatase Molecular biology Rats Resorption Staining Acetazolamide medicine.anatomical_structure chemistry Biochemistry Neutral Red Mechanics of Materials Microscopy Electron Scanning Ceramics and Composites biology.protein |
| Zdroj: | Biomaterials. 16:1327-1332 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/0142-9612(95)91048-4 |
| Popis: | The adherence of osteoblast-like cells and the resorption activity of isolated osteoclasts on calcium sulphate hemihydrate (CSH) was investigated. After a 24 h incubation period, alkaline phosphatase staining showed that rat osteoblast-like cells ROS 17/2.8 attached on CSH. Neutral red (NR) and tartrate-resistant acid phosphatase (TRAP) staining revealed that osteoclasts attached on CSH. Furthermore, osteoclasts formed lacunae as revealed by scanning electron microscopy. Although the lacunae formed by osteoclasts on CSH were diverse in shape and form, the most common type had approximately a circular outline, with a well-defined margin similar to those formed on dentine. Less commonly, excavations appeared as a discontinuous area of resorbed CSH with the presence of a circular zone around the non-resorbed area. Finally, by using 10(-9) M calcitonin, evidence was obtained that NR-positive cells were osteoclasts (58.3% and 57.66% decrease of NR-positive mouse and rat cells detected on CSH after 24 h incubation). However, no inhibition was obtained with 10(-11) M calcitonin. The overall number of NR-positive osteoclasts adherent on 256 mm2 CSH was 43 +/- 14 and 42 +/- 3 for mice and rat, respectively. The overall number of TRAP-positive mouse osteoclasts was 67 +/- 12. Acetazolamide (10(-5) M), a carbonic anhydrase inhibitor, inhibited the number of adherent NR osteoclasts on CSH by 50.42% and 41.6% for mouse and rat, respectively. These results indicate that osteoblasts attach on CSH and osteoclasts resorb CSH in vitro. |
| Databáze: | OpenAIRE |
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