Interaction of Antibiotics with Lipid Vesicles on Thin Film Porous Silicon Using Reflectance Interferometric Fourier Transform Spectroscopy
| Autor: | Cédric Godefroy, Frédérique Cunin, Pierre-Emmanuel Milhiet, Stephanie Pace, Taryn Guinan, Nicolas H. Voelcker, Nicole Lautredou |
|---|---|
| Přispěvatelé: | Mawson Institute, University of South Australia, INRA Unité 1054, Institut National de la Recherche Agronomique (INRA), Centre de Biochimie Structurale [Montpellier] (CBS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), French-Australian S&T FAST/EGIDE, Guinan, Taryn Monique, Godefroy, C, Lautredou, Nicole, Pace, Stephanie, Milhiet, PE, Voelcker, Nicolas, Cunin, F, University of South Australia [Adelaide], Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Silicon
[SDV.BIO]Life Sciences [q-bio]/Biotechnology Surface Properties Analytical chemistry Phospholipid 02 engineering and technology pharmaceuticals 010402 general chemistry Porous silicon 01 natural sciences Fourier transform spectroscopy antibiotics chemistry.chemical_compound Spectroscopy Fourier Transform Infrared Electrochemistry General Materials Science Crystalline silicon Particle Size [INFO.INFO-BT]Computer Science [cs]/Biotechnology Lipid bilayer Spectroscopy cell membranes Vesicle Surfaces and Interfaces [CHIM.MATE]Chemical Sciences/Material chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Lipids Anti-Bacterial Agents 0104 chemical sciences Membrane chemistry Chemical engineering Gramicidin lipids (amino acids peptides and proteins) Adsorption 0210 nano-technology Porosity Physical Chemistry (incl. Structural) |
| Zdroj: | Langmuir Langmuir, American Chemical Society, 2013, 29 (32), pp.10279−10286. ⟨10.1021/la401804e⟩ |
| ISSN: | 0743-7463 1520-5827 |
| DOI: | 10.1021/la401804e⟩ |
| Popis: | International audience; The ability to observe interactions of drugs with cell membranes is an important area in pharmaceutical research. However, these processes are often difficult to understand due to the dynamic nature of cell membranes. Therefore, artificial systems composed of lipids have been used to study membrane properties and their interaction with drugs. Here, lipid vesicle adsorption, rupture, and formation of planar lipid bilayers induced by various antibiotics (surfactin, azithromycin, gramicidin, melittin and ciprofloxacin) and the detergent dodecyl-b-D-thiomaltoside (DOTM) was studied using reflective interferometric Fourier transform spectroscopy (RIFTS) on an oxidized porous silicon (pSi) surface as a transducer. The pSi transducer surfaces are prepared as thin films of 3 μm thickness with pore dimensions of a few nanometers in diameter by electrochemical etching of crystalline silicon followed by passivation with a thermal oxide layer. Furthermore, the sensitivity of RIFTS was investigated using three different concentrations of surfactin. Complementary techniques including atomic force microscopy, fluorescence recovery after photobleaching, and fluorescence microscopy were used to validate the RIFTS-based method and confirm adsorption and consequent rupture of vesicles to form a phospholipid bilayer upon the addition of antibiotics. The method provides a sensitive and real-time approach to monitor the antibiotic-induced transition of lipid vesicles to phospholipid bilayers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|