Osmotic stress is accompanied by protein glycation inArabidopsis thaliana
| Autor: | Robert Berger, Wolfgang Brandt, Rico Schmidt, Elena Tarakhovskaya, Thomas Vogt, Stefanie Stöckhardt, Ludger A. Wessjohann, Andrea Sinz, Gagan Paudel, Klaus Humbeck, Claudia Birkemeyer, Uta Greifenhagen, Gerd Ulrich Balcke, Tatiana Bilova, Andrej Frolov |
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| Rok vydání: | 2016 |
| Předmět: |
Glycation End Products
Advanced 0301 basic medicine Glycosylation Arabidopsis thaliana Proteome Osmotic shock two-dimensional chromatography Physiology Arabidopsis Plant Science food quality label-free quantification 03 medical and health sciences chemistry.chemical_compound Osmotic Pressure Glycation Amadori rearrangement Botany Advanced glycation end-products (AGEs) Dehydration biology crop quality Arabidopsis Proteins drought stress Monosaccharides plant proteomics food and beverages biology.organism_classification 030104 developmental biology chemistry Biochemistry Osmolyte Plant protein glycation Transcriptome Oxidation-Reduction Research Paper |
| Zdroj: | Journal of Experimental Botany Scopus-Elsevier |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/erw395 |
| Popis: | Highlight Osmotic stress enhances the rate of protein glycation and monosaccharide autoxidation is the main pathway. Among the environmental alterations accompanying oncoming climate changes, drought is the most important factor influencing crop plant productivity. In plants, water deficit ultimately results in the development of oxidative stress and accumulation of osmolytes (e.g. amino acids and carbohydrates) in all tissues. Up-regulation of sugar biosynthesis in parallel to the increasing overproduction of reactive oxygen species (ROS) might enhance protein glycation, i.e. interaction of carbonyl compounds, reducing sugars and α-dicarbonyls with lysyl and arginyl side-chains yielding early (Amadori and Heyns compounds) and advanced glycation end-products (AGEs). Although the constitutive plant protein glycation patterns were characterized recently, the effects of environmental stress on AGE formation are unknown so far. To fill this gap, we present here a comprehensive in-depth study of the changes in Arabidopsis thaliana advanced glycated proteome related to osmotic stress. A 3 d application of osmotic stress revealed 31 stress-specifically and 12 differentially AGE-modified proteins, representing altogether 56 advanced glycation sites. Based on proteomic and metabolomic results, in combination with biochemical, enzymatic and gene expression analysis, we propose monosaccharide autoxidation as the main stress-related glycation mechanism, and glyoxal as the major glycation agent in plants subjected to drought. |
| Databáze: | OpenAIRE |
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