Prolactin Modifies Follicle-Stimulating Hormone-Induced Prostaglandin Synthesis by the Rat Granulosa Cell
| Autor: | R A, Knazek, R J, Christy, K C, Watson, M F, Lim, P N, Van Gorder, J R, Dave, L L, Richardson, S C, Liu |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
endocrine system
medicine.medical_specialty Gonadotropins Equine medicine.drug_class Granulosa cell Indomethacin Diethylstilbestrol Biology Dinoprost Chorionic Gonadotropin Follicle-stimulating hormone Endocrinology Internal medicine medicine Animals Ovarian follicle Progesterone Granulosa Cells Dose-Response Relationship Drug Prostaglandins E Prostaglandins F Rats Inbred Strains Hair follicle Prolactin Rats medicine.anatomical_structure Prostaglandins Female Follicle Stimulating Hormone Gonadotropin hormones hormone substitutes and hormone antagonists medicine.drug Hormone |
| Zdroj: | Endocrinology. 109:1566-1572 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo-109-5-1566 |
| Popis: | Female rats that had been hypophysectomized and implanted with a diethylstilbestrol capsule at 21 days of age were sacrificed 7 days after treatment with various combinations of ovine FSH, PMS gonadotropin, hCG, and ovine PRL (oPRL). Suspensions of granulosa cells obtained from the different groups were incubated at 37 C for 2 h with or without 5 × 10-4 M indomethacin. Prostaglandins (PG) E and F2α extracted from the suspensions and partially purified by chromatography on silicic acid columns were quantitated by specific RIA. The amounts of PG synthesized by the diethylstilbestrol-treated controls ranged from 10–2000 pg/106 cells and were not significantly altered by treatments with a single 5-IU hCG injection and/or 100 μg oPRL every 4 h. Three injections of 100 μg FSH markedly increased the rates of synthesis of PGE and PGF2α up to 2400 and 5600 pg/106 cells, respectively. These rates were increased still further by the injection of a FSH and hCG mixture. Additional injections of 100 μg oPRL every 4 h d... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|