Uncommon Ehrlichia canis infection associated with morulae in neutrophils from naturally infected dogs in Brazil
| Autor: | Bruna Marques dos Santos, Lívia Saab Muraro, Fernanda Panseri Rodrigues, Valéria Dutra, Daniel Moura de Aguiar, Márcio Garcia Ribeiro, Luciano Nakazato, Rafael Felipe da Costa Vieira, Regina Kiomi Takahira, Isis Indaiara Gonçalves Granjeiro Taques, Amanda Noéli da Silva Campos |
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| Přispěvatelé: | Federal University of Mato Grosso (UFMT), Universidade Estadual Paulista (Unesp), University of Cuiabá (UNIC), Universidade Federal do Paraná (UFPR) |
| Rok vydání: | 2018 |
| Předmět: |
Anaplasma platys
Male Anaplasmosis Ehrlichia ewingii canine ehrlichiosis 040301 veterinary sciences Ehrlichia canis Neutrophils animal diseases Ehrlichia Microbiology 0403 veterinary science 03 medical and health sciences Dogs parasitic diseases Animals Anaplasma Dog Diseases 030304 developmental biology 0303 health sciences General Veterinary General Immunology and Microbiology biology Coinfection Ehrlichiosis 04 agricultural and veterinary sciences General Medicine bacterial infections and mycoses biology.organism_classification Anaplasma phagocytophilum Tick-Borne Diseases Ehrlichiosis (canine) bacteria Female Hepatozoon canis Nested polymerase chain reaction Brazil |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1865-1682 |
| Popis: | Made available in DSpace on 2020-12-12T02:29:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-07-01 Ehrlichia and Anaplasma species are the most common tick-borne disease (TBD) pathogens in dogs worldwide. Ehrlichia canis, the aetiological agent of the Canine Monocytic Ehrlichiosis (CME), is known to replicate within the cytoplasm of mononuclear cells into clusters of organisms called morulae. However, detection of morulae in neutrophils is commonly observed in dogs infected by Ehrlichia ewingii or Anaplasma phagocytophilum. We report uncommon clinical cases of canine ehrlichiosis presenting morulae compatible with E. ewingii and A. phagocytophilum in dogs from two distinct regions of Brazil. Eight dogs were admitted to two veterinary teaching hospitals from Brazil, showing clinical or haematological signs suggestive of TBD. Blood or peritoneal fluid was withdrawn for haematological and cytologic analysis. All samples were evaluated by PCR assays for Ehrlichia and Anaplasma using genus-specific primers for dsb, 16S rRNA and groEL genes, followed by sequencing. Samples were also evaluated by nested PCR assays for the 16S rRNA gene of E. ewingii and groEL gene of A. phagocytophilum and Anaplasma platys. Seven dogs revealed thrombocytopenia, six dogs had monocytosis and five presented lymphopenia and anaemia. All dogs showed morulae structures compatible with Ehrlichia spp. in neutrophils and were PCR-positive for the dsb and 16S rRNA gene fragments of Ehrlichia, with sequences showing 100% identity with multiple E. canis sequences deposited in the GenBank™. Sequencing of 16S rRNA and groEL gene fragments from one PCR-positive dog showed 100% identity with A. platys. Overall, our data suggest that in endemic regions for E. canis, that is Brazil, the presence of morulae in neutrophils may indicate infection by this bacterium. Herein, morulae were also found in neutrophils present in the peritoneal fluid of a dog. Also, this is the first report of E. canis and Hepatozoon canis co-infection in neutrophils from naturally infected dogs confirmed by DNA sequencing. Laboratory of Virology and Rickettsial Infections Veterinary Hospital Federal University of Mato Grosso (UFMT) Veterinary Clinical Laboratory Department of Veterinary Clinics São Paulo State University (UNESP) Department of Veterinary Hygiene and Public Health São Paulo State University (UNESP) Laboratory of Clinical Pathology Veterinary Medicine College University of Cuiabá (UNIC) Laboratory of Microbiology and Molecular Biology Veterinary Hospital of the Faculty of Veterinary Medicine Federal University of Mato Grosso (UFMT) Department of Veterinary Medicine Federal University of Paraná (UFPR) Veterinary Clinical Laboratory Department of Veterinary Clinics São Paulo State University (UNESP) Department of Veterinary Hygiene and Public Health São Paulo State University (UNESP) |
| Databáze: | OpenAIRE |
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