Misaponin B Induces G2/M Arrest, Cytokinesis Failure and Impairs Autophagy
| Autor: | Ji Hoon Jung, Sung-Hoon Kim, Ji Eon Park, Gunho Won, Eun Jung Sohn, Hyo-Jung Lee, Hyung Jin Kim, Bum Sang Shim |
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| Rok vydání: | 2019 |
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Article Subject
Vacuole General Biochemistry Genetics and Molecular Biology Fluorescence Cell Line Tumor Autophagy Humans Cytotoxicity Cytokinesis A549 cell General Immunology and Microbiology Cell Death Chemistry Autophagosomes General Medicine Transfection Saponins Cell biology G2 Phase Cell Cycle Checkpoints Molecular Weight Cell culture Cancer cell Medicine M Phase Cell Cycle Checkpoints Microtubule-Associated Proteins Biomarkers Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2020 (2020) |
| ISSN: | 2314-6141 |
| Popis: | Saponins are a group of naturally occurring plant glycosides with the features of their strong foam-forming properties and multibiological effects such as antitumor activity. Though Misaponin B, one of the triterpenoid saponins from Madhuca longifolia, is known to have spermicidal and antioxidant activity, the other biological activities have been never reported so far. Thus, in the present study, the antitumor mechanism of Misaponin B was investigated in A549 and AsPC-1 cancer cells. Misaponin B exerted significant cytotoxicity in A549, H460, SKOV3, and AsPC-1 cancer cells. Among them, A549 and AsPC-1 cells were more susceptible to Misaponin B. Misaponin B induced G2/M arrest and cytokinesis failure and increased the expression of LC3B and p62 with autophagic vacuoles and GFP-LC3 punctae in A549 and AsPC-1 cells. Furthermore, Misaponin B suppressed autophagy flux in A549 cells transfected by GFP-mRFP-LC3 constructs by showing merged yellow color by autophagy flux assay. Overall, our findings provide evidences that Misaponin B induces G2M arrest and impairs autophagy in A549 and AsPC-1 cells. |
| Databáze: | OpenAIRE |
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